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A Study on Remediation of Diesel-Contaminated Soil by Biosurfactant- Enhanced Soil Washing  

문혜준 (명지대학교 청정기술원, 환경생물공학과)
임영경 (명지대학교 청정기술원, 환경생물공학과)
김윤관 (명지대학교 청정기술원, 환경생물공학과)
주춘성 (명지대학교 청정기술원, 환경생물공학과)
방기연 (명지대학교 청정기술원, 환경생물공학과)
정욱진 (명지대학교 청정기술원, 환경생물공학과)
이승우 (에코솔루션)
Publication Information
Journal of Soil and Groundwater Environment / v.7, no.2, 2002 , pp. 13-22 More about this Journal
Abstract
Soil washing by surfactants is a technology to enhance mobilization and subsequent degradation of oil pollutants by reducing the surface tension of pollutants which is combined with soil. In this study, biosurfactant, rhamnolipid was produced from Pseudomonas aemginosa ATCC 9027 which had an excellent biodegradable activity in soil without causing secondary pollution. Effects of chemical surfactants on the removal of diesel from diesel-contaminated soil were compared to those of biosurfactants including rhamnolipid. Diesel removal efficiency by rhamnolipid extracted from P. aeruginosa culture broth was over 95% in both batch and column washing test in 5,000ppm diesel-contaminated soil with 1% surfactants after washing for 24 hours. On the contrary, the results of chemical surfactants were below 50∼80%, The chemical surfactants with HLB value(8∼15) showed more then 75% efficiency of diesel removal. But, when the HLB values were below 8 or over 15. their efficiency were observed as less then 60% of diesel removal. Rhamnolipid, biologically produced surfactants, may also be promising agent for enhancing diesel removal from contaminated soil.
Keywords
rhamnolipid; Pseudomonas aeruginosa 9027; surfactant; biosurfactant; soil washing;
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1 '환경오염공정시험법', 동화기술 (1996)
2 이광래 외 '최신환경공학', 동화기술, pp639-666 (2001)
3 Hisatsuka K., Nakahara T., Minoda Y., and Yamada K. 'Formation of protein-like activator for n-alkane oxidation and its properties', Agr. Biol. Chem., 41, pp445-450(1976)
4 Chang H. P., Son C. K., Kim S. H., and Ahn D. G. 'Effect of pH and temperature on the production of biosurfactant by Pseudomonas aeruginosa YPJ-80 and its separation', Korean J. Biotechnol. Bioeng., 13, 5, pp511-517 (1998)
5 Itoh, S. and Honda H. 'Rhamnolipid produced by Pseudomonas aeruginosa, grown on n-paraffin', J. Anbibio., 24(12),pp855-859 (1971)
6 Abdul A. S. and Gibson T. L. 'Laboratory studies of studies of surfactant- enhanced washing of polychlorinated biphenyl from sandy material', Environ. Sci. Technol. 25, pp665-671 (1991)   DOI
7 Aronstein B. N., Calvillo Y. M., and Alexander M. 'Effect of surfactants at low concentrations on the desorption and biodegradation of sorbed aromatic compounds in soil', Environ. Sci. Tchnol. 25, pp1728-1731 (1991)   DOI
8 Michele I. V. D., Gulley S. L., Lee H., and Trevors J. T. 'Evaluation of microbial surfactants for recovery of hydrophobic pollutants from soil' , J. Indus. Microbiol, 11, pp163-170 (1993)   DOI   ScienceOn
9 David S. B. and Judith L. S. 'Ground Water Issue : Fundamentals of Soil Science as Applicable to Management of Hazardous Wastes', U.S. EPA, EPA /540/S-98/500, April (1999)
10 Yimin Z. 'Enhanced Octadecane Dispersion and Biodegradation by a Pseudomonas Rhamnolipid Surfactant (Biosurfactanat)', Appl Environ. Microbiol., 58(10),pp3276-3282 (1992)
11 김대원, 김민주, 강상모, 'Pseudomonas aeruginosa 가 생산하는 biosurfactant의 분리 및 특성' , Kor. J. Appl. Microbiol. Biotechnol. 23(3), pp337-345 (1995)
12 Jitendra D., Desai and Ibrahim M. B. 'Microbial production of surfactants and their commercial potendal', Microbiol. and Mol. Bio. Rev. 61(1), pp47-64(1997)
13 Hisatsuka, K., Nagahara T., and Yamada K. 'Protein like activator for alkane oxidation by Pseudomonas aeruginosa S7B1', Agri. Biol. Chem. 36, pp1361-1369 (1972)   DOI
14 박용하, 이승의 '토양환경 보전을 위한 오염방지기준 및 관리대책', 한국환경기술개발원, pp131 (1995)
15 최상일 외 '토양세척기법을 이용한 오염토양 정화기술의 적용성 실험', 삼성물산(주) 건설부문 기술연구소 보고서 (1996)
16 Harvey S., Elashvilli I., Valdes J. J., Kamely D., and Chakrabarty A. M. 'Enhanced removal of Exxon Valdez spilled oil from Alaskan gravel by a microbial surfactant' Bio/Technol., 8, pp228-230 (1990)   DOI   ScienceOn
17 Geroge G., Lin S. C., and Sharma M. M. 'Surface-active compounds from microorganisms', Biol Technol., 10, pp60-65 (1992)   DOI   ScienceOn
18 Ellis W. D., Payne J. R., and McNabb G. D. 'Treatment of contaminated soils with aqueous surfactants' U. S. Environ. Prot. Agency Res. Dev. Rep. No. EPA/600/S2-85/129 (1985)
19 Batu P. S., Vaidya A. N., Bal A. S., Kapur R., Juwarkar A., and Khanna P. 'Kinetics of biosurfactant production by Pseudomonas aeruginosa strain BS2 from industrial wastes', Biotech. Lett. 18 (3), pp263-268 (1996)
20 Harwell J. H. 'Factors affecting surfactant performance in groundwater remediation applications', Transport and Remediation of Subsurface Contaminants, Chapter 10,ACS(1992)
21 West C. C. and Harwell J. H. 'Surfactants and subsurface remediation', Environ. Sci. Technol, 26, pp 2324-2330(1992)   DOI
22 최정 외 '토양학실험', 형설출판사 (1994)
23 Nyer E. K. 'Practical techniques for groundwater and soil remediation', Lewis Publishers, pp1-42 (1993)