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Surface Immobilization of Amphiphilic Comb-like Polymer on Polydimethylsiloxane and in vitro Cytotoxicity Assay  

Choi, Jaeyoo (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
Jung, Jaeyeon (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
Cheng, Jie (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
Lee, Jonghwan (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
Hyun, Jinho (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
Kim, Hyunjoong (Lab of Adhesion and Bio-Composites, Program in Environmental Materials Science, Seoul National University)
Publication Information
Journal of Adhesion and Interface / v.11, no.2, 2010 , pp. 70-75 More about this Journal
Abstract
It described the modification of polydimethylsiloxane (PDMS) with amphiphilic methyl methacrylate-based polyethylene glycol (PMMA-b-PEG) to enhance the hydrophilicity of a PDMS surface and cytotoxicity of it. PMMA-b-PEG solutions in water/ethanol mixture was spun-cast on the PDMS surface and the surface was characterized by long-term measurement of water contact angle. The morphology of PDMS surfaces coated with PMMA-b-PEG was characterized by field emission scanning electron microscopy and atomic force microscope. Cytotoxicity of the modified surfaces was investigated by MTT assay which would be necessary for the evaluation of tissue compatibility after implantation of the materials. Based on the MTT assay, PDMS coated with PMMA-b-PEG didn't show any significant cytotoxcity.
Keywords
Polydimethylsiloxane (PDMS); cytotoxicity; amphiphilic polymer; methyl methacrylate-based polyethylene glycol (PMMA-b-PEG); MTT assay;
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