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http://dx.doi.org/10.6564/JKMRS.2013.17.2.076

NMR Study of Consensus DNA-binding Site for Arabidopsis thaliana Class I Transcription Factor AtTCP1  

Choi, Yong-Geun (Department of Chemistry and RINS, Gyeongsang National University)
Kim, Hee-Eun (Department of Chemistry and RINS, Gyeongsang National University)
Lee, Joon-Hwa (Department of Chemistry and RINS, Gyeongsang National University)
Publication Information
Journal of the Korean Magnetic Resonance Society / v.17, no.2, 2013 , pp. 76-80 More about this Journal
Abstract
The TCP domain is a DNA-binding domain present in plant transcription factors and has a similar structural feature to the bHTH motif of eukaryotic transcription factors. The imino proton exchange study has been performed for the DNA duplex containing the consensus DNA-binding site for the AtTCP11 transcription factor. The first two base pairs in the consensus 5'-GTGGG-3' sequence are relatively very unstable but lead to greater stabilization of the neighboring two G C base pairs. These unique dynamic features of the five base pairs in the consensus DNA sequence might play crucial roles in the effective DNA binding of the AtTCP11 protein.
Keywords
NMR; DNA binding; Hydrogen exchange; TCP transcription factor;
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