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http://dx.doi.org/10.5660/WTS.2015.4.3.225

Discrimination of Echinochloa colona (L.) Link from other Echinochloa Species using DNA Barcode  

Lee, Jeongran (National Academy of Agricultural Science)
Kim, Chang-Seok (National Academy of Agricultural Science)
Lee, In-Yong (National Academy of Agricultural Science)
Publication Information
Weed & Turfgrass Science / v.4, no.3, 2015 , pp. 225-229 More about this Journal
Abstract
Echinochloa colona is one of the most problematic weeds in the paddy fields of the world. In recent years, this species is likely to be introduced in Korea due to global warming, the expansion of international trade including agricultural products, and increasing tourists. We tried to identify the species from Korean Echinochloa crus-galli and E. oryzicola in order to establish the control measures in case of the initial influx. For this study, Echinochloa colona collected from the National Plant Germplasm System, USA were examined and E. crus-galli and E. oryzicola were collected in Korea. It is, however, very difficult to identify for Echinochloa species using morphological characters because of numerous interspecific and intraspecific types found in nature. Thus, we barcoded the species using rbcL, matK, and ITS. All three markers identified E. colona very well from the others. ITS alone may be enough as a DNA barcode for E. colona identification, when considering cost and effectiveness. The barcode sequences were deposited to the National Center for Biotechnology Information database for public use.
Keywords
DNA barcode; Echinochloa colona; E. crus-galli; E. oryzicola; Identification;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 Barrett, S.C.H. 1983. Crop mimicry in weeds. Econ. Bot. 37:255-282.   DOI
2 Chase, M.W. and Fay, M.F. 2009. Barcoding of plants and fungi. Sci. 325:682-683.   DOI
3 Chen, S. and Peterson, P.M. 2006. Poaceae (Gramineae). Vol. 22, Poaceae (Graminae), pp. 515-518. In: Wu et al. (Eds.). Flora of China. Science Press, Beijing, China and Missouri Botanical Garden Press St. Louis, USA.
4 Chen, S., Yao, H., Han, J., Liu, C., Song, J., et al. 2010. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS ONE 5:e8613(8611-8618).
5 China Plant BOL Group. 2011. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants. P. Natl. Acad. Sci. USA. 108:19641-19646.   DOI
6 Clayton, W.D. and Renvoize, S.A. 1999. Genera graminum, grasses of the world. London: Royal Botanic Gardens, Kew. pp. 280-281.
7 Hebert, P.D.N., Cywinska, A., Ball, S.L. and Waard, J.R.d. 2003. Biological identifications through DNA barcodes. P. R. Soc. London, Series B. 270:313-321.   DOI
8 Holm, L.G., Plucknett, D.L., Pancho, J.V. and Herberger, J.P. 1977. The world's worst weeds: Distribution and biology. The University Press of Hawaii, Honolulu, USA. pp. 32-46.
9 Kress, W.J. and Erickson, D.L. 2007. A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS ONE 2:e508.   DOI
10 Lahaye, R., Bank, M.v.d., Bogarin, D., Warner, J., Pupulin, F., et al. 2008. DNA barcoding the floras of biodiversity hotspots. P. Natl. Acad. Sci. USA. 105:2923-2928.   DOI
11 Lee, J., Kim, C.S. and Lee, I.Y. 2015. Molecular identification of Pooideae, Poaceae in Korea. Weed Turf. Sci. 4:18-25. (In Korean)   DOI
12 Lee, J., Kim, C.S. and Lee, I.Y. 2014a. Evaluating the discriminatory power of DNA barcodes in Panicoideae, Poaceae. J. Agric. Sci. Technol. B 4:533-544.
13 Mennan, H. and Kaya-Altop, E. 2012. Molecular techniques for discrimination of late watergrass (Echinochloa oryzicola) and early watergrass (Echinochloa oryzoides) species in Turkish rice production. Weed Sci. 60:525-530.   DOI
14 Lee, J., Kim, C.S. and Lee, I.Y. 2014b. Taxonomic review of the genus Echinochloa in Korea (I): inferred from sequences of cpDNA and nrDNA. Weed Turf. Sci. 3:183-189.   DOI
15 Lee, J., Kim, C.S. and Lee, I.Y. 2014c. Taxonomic review of the genus Echinochloa in Korea (II): inferred from simple sequence repeats. Weed Turf. Sci. 3:190-195.   DOI
16 Lee, J., Kim, C.S. and Lee, I.Y. 2013. Identification of Echinochloa oryzicola (Vasinger) Vasinger and E. oryzoides (Ard.) Fritsch in Korea. Kor. J. Pla. Tax. 43:56-62. (In Korean)   DOI
17 Michael, P.W. 2007. Echinochloa P. Beauv. Vol. 25, Magnoliophyta: Commelinidae (in part): Poaceae, part 2. pp. 390-403. In: Barkworth et al. (Eds.). Flora of North America, North of Mexico. Oxford University Press, New York, USA.
18 Oh, S.M., Kim, C.S., Moon, B.C. and Lee, I.Y. 2002. Inflow information and habitat current status of exotic weeds in Korea. Kor. J. Weed Sci. 22:280-295. (In Korean)
19 Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
20 Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30:2725-2729.   DOI   ScienceOn
21 Warwick, S.I. and Weaver, S.E. 1980. Atrazine resistance in Amaranthus retroflexus (redroot pigweed) and Amaranthus powellii (green pigweed) from southern Ontario. Can. J. Plant Sci. 60:1485-1488.   DOI