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http://dx.doi.org/10.5660/WTS.2015.4.2.124

Soil Microbial Community Analysis in Large Patch (Rhizoctonia solani AG2-2 IV)  

Lee, Jung Han (Korea Turfgrass Research Institute)
Min, Gyu Young (Daejung Golf Engineering Co. Ltd.)
Shim, Gyu Yul (Department of Plant Medicine and Institute of Agriculture and Life Science, Gyeonsang National University)
Jeon, Chang Wook (Southern Forest Resource Research Center, Korea Forest Research Institute)
Choi, Su min (Southern Forest Resource Research Center, Korea Forest Research Institute)
Han, Jeong Ji (Korea Turfgrass Research Institute)
Kwak, Youn-Sig (Department of Plant Medicine and Institute of Agriculture and Life Science, Gyeonsang National University)
Publication Information
Weed & Turfgrass Science / v.4, no.2, 2015 , pp. 124-128 More about this Journal
Abstract
Large patch, caused by Rhizoctonia solani AG2-2 IV, is a soil-born disease that is the most important of warm season turfgrass such as zoysia and Bermuda grass. This study was conducted to analysis of the soil microbial community structure on large patch. Center of the large patch (CLC), edge (CLE) and healthy (CLH) part of microbial communities were examined using metagenomics in Phylum level. Distribution trends of the rhizosphere microorganisms were similar to the order Proteobacteria, Acidobacteria, Chloroflexi, Firmicutes, Planctomycetes, Gemmatimonadetes, Nitrospira, Cyanobactria and Verrucomicrobia in soil collections. Contrastively Actinobacteria was more 56% abundant in healthy part soil (16%) than in the center (9.28%) or edge (10.84%) parts. Taxonomic distributions were compared among the CLC, CLE and CLH, total 6,948 OTUs were detected in the CLC, 6,505 OTUs for the CLE and 5,537 OTUs were detected in the CLE. Distributions of Actinobacteria OTUs were appeared 615 OTUs in the CLC, 709 OTUs in the CLE and 891 OTUs in the CLH. Among Actinobacteria, 382 OTUs were overlapped in the all soils. Not matched OTUs of CLH (286 OTUs) was detected 23 times higher than CLC (91 OTUs) and CLE (126 OTUs).
Keywords
Large Patch; Metagenomics; Rhizoctonia solani; Zoysiagrass;
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