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http://dx.doi.org/10.5660/KJWS.2010.30.4.361

Analytical Method for MCPA Residue in Brown Rice and Rice Straw by HPLC/UVD  

Yoo, Ki-Yong (Department of Food and Environment Science, College of Life Science and Natural Resources, Wonkwang University)
Kang, Dae-Won (Department of Food and Environment Science, College of Life Science and Natural Resources, Wonkwang University)
Choi, Yong-Hwa (School of Ecological & Environmental System, College of Ecological & Environmental Science, Kyungpook National University)
Han, Seong-Soo (Department of Food and Environment Science, College of Life Science and Natural Resources, Wonkwang University)
Publication Information
Korean Journal of Weed Science / v.30, no.4, 2010 , pp. 361-370 More about this Journal
Abstract
This study was carried out to establish the analytical method of MCPA residue in brown rice and rice straw by HPLC/UVD. When MCPA was extracted from sample under the pH 3.6 by adding acetone 200 mL and 1N-HCl 100 mL, the extraction efficiency was high by 87%. And purification efficiency was high by 83% when 5 mL of 1% methanol/acetonitrile was eluated by the florisil Sep-pak cartridge column. From spiking of $0.1{\mu}g\;mL^{-1}$ and $0.25{\mu}g\;mL^{-1}$ of MCPA to control sample, respectively, average recovery rate of MCPA in brown rice was 96.0% and 94.9% and that in rice straw was 92.5% and 88.2%, respectively. Precision of experiment was very high by relative standard deviation of 1.5% to 5.7%. In brown rice and rice straw treated with bentazone+MCPA (11+1.2%) of 30 kg and 60 kg per ha at 30 days after rice transplanting, respectively, maximum residue limit was under $0.05{\mu}g\;mL^{-1}$ of the recommended rate of Korean Food and Drug Administration. From the above results, the analytical procedure of MCPA in plants such as hydrolysis, saponification and derivatization were ommited, and retention time was faster and recovery rate was higher than the existed results of HPLC/UVD. Therefore, these results were greatly improved and seemed to be usefully applied for residue analysis of MCPA in plants.
Keywords
analytical method; MCPA; brown rice and rice straw; HPLC/UVD;
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