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http://dx.doi.org/10.5012/jkcs.2022.66.4.298

Induction of Apoptosis of DK-5-62, a Novel (-)-Catechin Derivative Through MAPKs Signaling Pathway in HCT116 Cells  

Guon, Tae Eun (College of Science and Biotechnology, Duksung Women's University)
Shin, Dong-Soo (Department of Chemistry, Changwon National University)
Chung, Ha Sook (College of Science and Biotechnology, Duksung Women's University)
Publication Information
Journal of the Korean Chemical Society / v.66, no.4, 2022 , pp. 298-304 More about this Journal
Abstract
The present study was designed to investigate the molecular mechanisms of DK-5-62, a novel (-)-catechin derivative on HCT116 human colorectal cancer cells. DK-5-62 inhibited the proliferation in dose- and time-dependent manner accompanied by the morphological changes. Effects of DK-5-62 appeared to be mediated by the induction of apoptosis, as manifested through DNA-binding dye Hoechst 33258 staining. Analysis of the mechanism of these events indicated that DK-5-62-treated cells exhibited an increased ratio of Bax/Bcl-2, resulting in the activation of caspase-9, caspase-3, and poly-ADP-ribose polymerase in a dose-dependent manner. Moreover, DK-5-62-induced apoptosis was accompanied by phosphorylation of the mitogen-activated protein kinase family, c-Jun N-terminal kinase, p38, and extracellular signal-regulated kinase. These results suggest that HCT116 cells are moderately sensitive to growth inhibition by DK-5-62 via apoptosis, as evidenced by activation of ERK/p38/Bcl-2 family signaling, as well as alteration in caspase-9 and caspase-3.
Keywords
(-)-catechin derivatives; Apoptosis; MAPKs; HCT116 cells;
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