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http://dx.doi.org/10.5012/jkcs.2010.54.5.573

Study of Interaction of Native DNA with Iron(III)-(2,4-Dihydroxysalophen)chloride  

Azani, Mohammad-Reza (Departamento de Quimica Inorganica, Facultad de Ciencias, Universidad Autonoma de Madrid)
Hassanpour, Azin (Departamento de Quimica Inorganica, Facultad de Ciencias, Universidad Autonoma de Madrid)
Bordbar, Abdol-Khalegh (Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan)
Publication Information
Journal of the Korean Chemical Society / v.54, no.5, 2010 , pp. 573-578 More about this Journal
Abstract
In this study, iron(III)-2,4-dihydroxysalophen chloride (Fe(2,4-DHSalophen)Cl), has been synthesized by combination of 2,4-dihydroxysalophen (2,4-DHSalophen) with $FeCl_2$ in a solvent system. This complex combination was characterized using UV-vis and IR spectroscopies. Subsequently, the interaction between native calf thymus deoxyribonucleic acid (ct-DNA) and Fe(2,4-DHSalophen)Cl, was investigated in 10 mM Tris/HCl buffer solution, pH 7.2, using UV-visible absorption and fluorescence spectroscopies, thermal denaturation technique and viscosity measurements. From spectrophotometric titration experiments, the binding constant of Fe(2,4-DHSalophen)Cl with ct-DNA was found to be $(1.6{\pm}0.2){\times}10^3\;M^{-1}$. The fluorescence study represents the quenching effect of Fe(2,4-DHSalophen)Cl on bound ethidium bromide to DNA. The quenching process obeys linear Stern-Volmer equation in extended range of Fe(2,4-DHSalophen)Cl concentration. Thermal denaturation experiments represent the increasing melting temperature of DNA (about $4.3^{\circ}C$) due to binding of Fe(2,4-DHSalophen)Cl. These results are consistent with a binding mode dominated by interactions with the groove of ct-DNA.
Keywords
DNA; Schiff base; Interaction; Fluorescence; UV-vis spectroscopy;
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