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http://dx.doi.org/10.5012/jkcs.2010.54.4.451

Heavy Metal Ion Detection in Living Cell Using Fluorescent Chemosensor  

Kwon, Pil-Seung (Department of Clinical Laboratory Science, Wonkwang Health Science University)
Kim, Jin-Kyung (Department of Miocrobiology & Vestibulocochlear Research Center, Wonkwang University College of Medicine)
Kim, Jong-Wan (Department of Laboratory Medicine, Dankook University College of Medicine)
Publication Information
Journal of the Korean Chemical Society / v.54, no.4, 2010 , pp. 451-459 More about this Journal
Abstract
The fluorescence detection of intracellular metal ions are high interest in the fields of organic molecular chemistry and cellular biology. This study was purposed to detection for mercury and zinc in the cell using fluorescent chemosensor (FS). FS exhibits a weak fluorescence, but emits strong fluorescence upon Zn$^{2+}$ complexation. The increased fluorescence of the 2FS/Zn$^{2+}$ can be quenched completely by addition of only 1 equiv of Hg$^{2+}$ with the formation of complex FS-Hg$^{2+}$. Four cell lines (LLC-MK2, Hela, HT29 and AMC-HN3) were used for fluorescence imaging by confocal microscope. The cell viability MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was evaluated after cell treatment of FS, Zn$^{2+}$, FS-Zn$^{2+}$, Hg$^{2+}$ on LLC-MK2 cell line. The cytotoxicity of FS was showed to viability over 80%. This study has shown that FS can be detected for selective imaging of Zn$^{2+}$ and Hg$^{2+}$ in living cells.
Keywords
Fluorescent chemosensor; Mercury; Zinc;
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