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http://dx.doi.org/10.5012/jkcs.2010.54.02.215

Detection of Salmonella Using the Loop Mediated Isothermal Amplification and Real-time PCR  

Ahn, Young-Chang (Dept. of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue Regeneration Engneering (ITREN), Dankook University)
Cho, Min-Ho (Dept. of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue Regeneration Engneering (ITREN), Dankook University)
Yoon, Il-Kyu (Dept. of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue Regeneration Engneering (ITREN), Dankook University)
Jung, Duck-Hyun (Dept. of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue Regeneration Engneering (ITREN), Dankook University)
Lee, Eun-Young (Dept. of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue Regeneration Engneering (ITREN), Dankook University)
Kim, Jin-Ho (Dept. of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue Regeneration Engneering (ITREN), Dankook University)
Jang, Won-Cheoul (Dept. of Chemistry, School of Advanced Science and Basic Science Research Institute, Institute of Tissue Regeneration Engneering (ITREN), Dankook University)
Publication Information
Journal of the Korean Chemical Society / v.54, no.2, 2010 , pp. 215-221 More about this Journal
Abstract
Salmonella is an important food-and water-borne pathogen associated with acute gastrointestinal illnesses around the world. The most common serotypes isolated from humans are Salmonella enterica serotype Typhimurium (S. Typhimurium) and S. Enteritidis. Traditional detection methods for Salmonella are based on cultures using selective media and characterization of suspicious colonies by biochemical and serological tests. These methods are generally time-consuming and not so highly sensitive. Recently, the Loop Mediated Isothermal Amplification and real-time PCR has been used as a highly sensitive, specific, and rapid test for the presence of pathogenic bacteria. In this study, a LAMP and real-time PCR was used to detect S. Typhimurium and S. Enteritidis. We selected target genes, which were the in invA and a randomly cloned sequence specific for the genus Salmonella. With LAMP and real-time PCR, random sequence was detected from Salmonella spp, invA were detected from all strain of S. Typhimurium and S. Enteritidis. This assay indicate that the specificity, sensitivity and rapid of the LAMP and real-time PCR make them potentially valuable tools for detection of S. Typhimurium and S. Enteritidis.
Keywords
LAMP(Loop Mediated Isothermal Amplification); PCR(Polymerase Chain Reaction); Salmonella Typhimurium; Salmonella Enteritidis;
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