Browse > Article
http://dx.doi.org/10.5012/jkcs.2007.51.1.036

Detection of Hepatitis B Virus Using Micro-PCR and Real-Time PCR Methods  

Kang, Won (Dept. of Chemistry, School of Advanced Science and Basis Science Research Institute Dankook University)
Park, Sang-Bum (Dept. of Chemistry, School of Advanced Science and Basis Science Research Institute Dankook University)
Nam, Youn-Hyoung (Dept. of Chemistry, School of Advanced Science and Basis Science Research Institute Dankook University)
An, Young-Chang (Dept. of Chemistry, School of Advanced Science and Basis Science Research Institute Dankook University)
Lee, Sang-Hyun (Dept. of Chemistry, School of Advanced Science and Basis Science Research Institute Dankook University)
Jang, Won-Cheoul (Dept. of Chemistry, School of Advanced Science and Basis Science Research Institute Dankook University)
Park, Su-Min (Chungnam Animal Science Center GenetBio, Konyang Univ)
Kim, Jong-Wan (Dept. of Clinical Pathology, Dankook University Medical College)
Chong, Song-Chun (Samsung Techwin)
Publication Information
Journal of the Korean Chemical Society / v.51, no.1, 2007 , pp. 36-42 More about this Journal
Abstract
Hepatitis B is a serious public health problem leading to chronic infection and liver cancer. Quantitation of circulating hepatitis B virus (HBV) is important for monitoring disease progression and for assessing the response to antiviral therapy. In this study, by using Real-Time PCR and novel Micro-PCR assay method, we measured HBV concentration in the clinical sample. A total of 120 serum samples from patients with HBV infection collected was in Dankook university hospital to compare the detection limit, sensitivity, specificity and reproducibility of the two assay methods. These findings of this study suggest that Micro-PCR and Real-Time PCR assay methods are comparable to each other in there detection limit, sensitivity, and reproducibility for HBV DNA quantitation. However, Micro-PCR assay is more efficient than Real-Time PCR method, because Real-Time PCR is not so time - consuming, technically easy and need to reagent of a small quantity. It will be useful for rapid and reliable clinical diagnosis of HBV in many countries.
Keywords
Hepatitis B Virus; Real Time-PCR; Micro-PCR;
Citations & Related Records

Times Cited By SCOPUS : 3
연도 인용수 순위
1 Ruben Bonilla Guerrero, Lewis R. Roberts. The role of hepatitis B virus integrations in the pathogenesis of human hepatocellular carcinoma. Journal of Hepatology 2005, 42, 760   DOI   ScienceOn
2 Guy Vernet, Nathalie Tran. The DNA-Chip technology as a new molecular tool for the detection of HBV mutants. Journal of Clinical Virolog. 2005, 34, S49   DOI   ScienceOn
3 Stephen Locarnini, William S. Mason. Cellular and virological mechanisms of HBV drug resistance. Journal of Hepatology 2006, 44, 422   DOI   ScienceOn
4 Rodolfo H. Campos, Viviana A. Mbayed, Flavia G. Pineiro y Leone. Molecular epidemiology of hepatitis B virus in Latin America. Journal of Clinical Virology 2005, 34, S8   DOI   ScienceOn
5 Xiaodong Zhang, Hang Zhang and Lihong Ye. Effects of hepatitis B virus X protein on the development of liver cancer. Journal of Laboratory and Clinical Medicine 2006, 147, 58   DOI   ScienceOn
6 Alba Maria Ropero, M. Carolina Danovaro-Holliday,; Jon K. Andrus. Progress in vaccination against hepatitis B in the Americas. Journal of Clinical Virology 2005, 34, S14   DOI   ScienceOn
7 Faure, E. Alternative peptide-fusion proteins generated by out-of-frame mutations, just upstream ORFs or elongations in mutants of Human Hepatitis B Viruses Virus Research. 2006, 117, 185   DOI   ScienceOn
8 Sunchai Payungporn, Pisit Tangkijvanich, Pojchanad Jantaradsamee, Apiradee Theamboonlers, Yong Poovorawan. Simultaneous quantitation and genotyping of hepatitis B virus by real-time PCR and melting curve analysis. Journal of Virological Methods 2004, 120, 131   DOI   ScienceOn
9 Jim O'Mahony, Colin Hill. A real time PCR assay for the detection and quantitation of Mycobacterium avium subsp. paratuberculosis using SYBR Green and the Light Cycler. Journal of Microbiological Methods 2002, 51, 283   DOI   ScienceOn
10 Dixon, T. J.; Taggart, J. B.; George, S. G. Application of real time PCR determination to assess interanimal variabilities in CYP1A induction in the European flounder (Platichthys flesus). Marine Environmental Research 2002, 54, 267   DOI   ScienceOn
11 Martin E. Adelson, Melanie Feola, Jason Trama, Richard C. Tilton, Eli Mordechai. Simultaneous detection of herpes simplex virus types 1 and 2 by real-time PCR and Pyrosequencing. Journal of Clinical Virology 2005, 33, 25   DOI   ScienceOn
12 김재현, 최국선. Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus. 생물학전문연구정보센터(BRIC) BioWave 2003, 5, 2
13 Yoon-Kyoung Choa, Jintae Kima, Youngsun Lee, Young-A. Kim, Kak Namkoong, Heekyun Lim, Kwang W. Oha, Suhyeon Kim, Jungim Han, Chinsung Park, Y. Eugene, Pak, Chang-Seok Ki, Jong Rak Choi, Hyeon-Koon Myeong, Christopher Ko. Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus. Biosensors and Bioelectronics 2006, 21, 2161   DOI   ScienceOn
14 Garson, J. A.; Grant, P. R.; Ayliffe, U.; Ferns, R. B.; Tedder, R. S. Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and murine cytomegalovirus internal control. Journal of Virological Methods. 2005, 126, 207   DOI   ScienceOn
15 Ponsiano Ocama, MD, Christopher K. Opio, MD, William M. Lee, MD. Hepatitis B virus infection: Current status. The American Journal of Medicine 2005, 118, 1413.e15   DOI   ScienceOn
16 Chemin, I.; Tre po. C. Clinical impact of occult HBV infections. Journal of Clinical Virology 2005, 34, S15   DOI   ScienceOn
17 Barros, M. F.; Rodrigues, P. A.; Matias, S. R.; Carreira, L. M.; Machado-Caetano. J. A. Detection of hepatitis B virus core mutants by PCR-RFLP in chronically infected patients. Journal of Virological Methods 2004, 120, 125   DOI   ScienceOn
18 Shiou-Hwei Yeh, Ching-Yi Tsai, Jia-Horng Kao; Chun-Jen Liu, Ti-Jung Kuo, Ming-Wei Lin, Wen-Ling, Huang, Shu-Fen Lu, Jane Jih, Ding-Shinn Chen, Pei-Jer Chen. Quantification and genotyping of hepatitis B virus in a single reaction by real-time PCR and melting curve analysis. Journal of Hepatology 2004, 41, 659   DOI   ScienceOn
19 Watzinger, F.; Ebner, K.; Lion, T. Detection and monitoring of virus infections by real-time PCR. Molecular Aspects of Medicine. 2006, 27, 254   DOI   ScienceOn