Browse > Article
http://dx.doi.org/10.5012/jkcs.2006.50.3.216

Surface Modification of Liposomes Using Comblike Copolymer for Enhancing Stability in Blood Circulation  

Sin, Byeong-Cheol (한국화학연구원)
Song, Chung-Gil (성균관대학교)
Hwang, Tae-Won (한국화학연구원)
Seong, Ha-Su (한국화학연구원)
Park, Eun-Seok (성균관대학교)
Publication Information
Journal of the Korean Chemical Society / v.50, no.3, 2006 , pp. 216-223 More about this Journal
Abstract
To increase the stability of liposomes in blood circulation, surface modification of liposomes by incorporating a lipid-polymer derivative in the lipid bilayer or conjugating a hydrophilic polymer to the liposomal surface has been developed. In this study, the comblike copolymer, poly(HEMA-co-HPOEM), having multiple polyethyleneoxide side chains was prepared by free radical polymerization of hydroxyethylmethacrylate (HEMA) and hydroxypolyoxyethylenemethacrylate (HPOEM) as vinyl monomers. Poly(HEMA-co-HPOEM) was conjugated to the liposomal surface and the characteristics of the modified liposomes in serum were investigated. Conjugation of poly(HEMA-co-HPOEM) to liposomes increased the particle size of the liposomes by 30 nm and decreased the absolute value of zeta potential of the liposomes by shielding the negative charge of liposomal surface. Loading efficiency of model drug, doxorubicin, in liposomes was about 90% and the efficiency was not affected by conjugation of poly(HEMA-co-HPOEM) to liposomes. The particle size of poly(HEMA-co-HPOEM)-conjugated liposomes in serum did not changed and the protein adsorption was lower than that of control liposomes or liposomes containing polyethyleneoxide-lipid derivative (PEG-liposomes). These results suggest that poly(HEMA-co-HPOEM) is efficient for the stabilization of liposomes in blood circulation.
Keywords
Liposome; poly(HEMA-co-HPOEM); Stability; Protein Adsorption
Citations & Related Records

Times Cited By SCOPUS : 1
연도 인용수 순위
1 Moghimi, S. M.; Szebeni, J. Prog. Lipid Res. 2003, 42, 463   DOI   ScienceOn
2 Klibanov, A. L.; Maruyama, K.; Torchilin, V. P.; Huang, L. FEBS Lett. 1990, 268(1), 235   DOI   ScienceOn
3 Moghimi, S. M.; Patel, H. M. Adv. Drug Delivery. Rev. 1998, 32, 45   DOI   ScienceOn
4 Darrell, J. I.; Anne, M. M. Biomacromolecules 2001, 2, 85   DOI   ScienceOn
5 Larsson, C. F.; Nylander, T.; Jannasch, P.; Wesslen, B. Biomaterials 1996, 17, 2199   DOI   ScienceOn
6 Nath, N.; Hyun, J.; Ma, H.; Chilkoti, A. Surface Sci. 2004, 570, 98   DOI   ScienceOn
7 Lawrence, M. J.; Lawrence, S. M.; Chauhan, S.; Barlow, D. J. Chem. Phys. Lipids 1996, 82, 89   DOI   ScienceOn
8 Auguste, D. T.; Prud'homme, R. K.; Ahl, P. L.; Meers, P.; Kohn, J. Biochim. Biophys. Acta 2003, 1616, 184   DOI   ScienceOn
9 Heitz, C.; Pendharker, S.; Prud'homme, R. K.; Kohn, J. Macromolecules 1999, 32, 6652   DOI   ScienceOn
10 Teodorescu, M. Eur. Polym. J. 2002, 38, 841   DOI   ScienceOn
11 Torchilin, V. P. Nat. Rev. Drug Discov. 2005, 4, 145   DOI   ScienceOn
12 Bangham, A. D.; Standish, M. M.; Watkis, J. C. J. Mol. Biol. 1965, 13, 238   DOI
13 Gregoriadis, G.; Florence, A. T. Drugs 1993, 45, 15
14 Han, H. D.; Shin, B. C.; Choi, H. S. Eur. J. Pharm. Biopharm. 2006, 62, 110   DOI   ScienceOn
15 Gabizon, A.; Papahadjopoulos, D. Proc. Natl. Acad. Sci. 1988, 85, 6949   DOI   ScienceOn
16 Che, B.; Winterhalter, M.; Frederick, P. M.; Vallner, J. J; Lasic, D. D. Adv. Drug Delivery. Rev. 1997, 24, 65
17 Lin, W.; Garnet, M. C; Davies, M. C; Bignotti, F.; Ferruti, P.; Davis, S. S.; Illum, L. Biomaterials 1997, 18, 559   DOI   ScienceOn
18 Haran, G.; Cohen, R.; Bar, L. K.; Barenholz, Y. Biochim. Biophys. Acta 1993, 1151, 201   DOI   ScienceOn
19 Takeuchi, H.; Kojima, H.; Toyoda, T.; Yamamoto, H.; Hino, T.; Kawashima, Y. Eur. J. Pharm. Biopharm. 1999, 48, 123   DOI   ScienceOn
20 Yoshida, A.; Hashizaki, K.; Yamauchi, H.; Sakai, H.; Yokoyama, S.; Abe, M. Langmuir 1999, 15, 2333   DOI   ScienceOn
21 Harashima, H.; Kiwada, H. Adv. Drug Delivery. Rev. 1996, 19, 425   DOI   ScienceOn
22 Nagayasu, A.; Uchiyama, K.; Kiwada, H. Adv. Drug Delivery. Rev. 1999, 40, 75   DOI   ScienceOn
23 Patel, H. M. Crit. Rev. Ther. Drug Carrier Syst. 1992, 9(1), 39
24 Baumgarten, H. J. Immunol. Methods 1985, 82(1), 25   DOI   ScienceOn