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http://dx.doi.org/10.5012/jkcs.2003.47.2.127

Diagnosis of Bovine Theileriosis by Direct PCR and Electrophoresis from Whole Blood Without DNA Extraction  

Seong Ho Kang (Department of Chemistry, Chonbuk National University)
Sangmin Jang (Department of Chemistry, Chonbuk National University)
Joon-Seok Chae (Bio-safety Research Institute, Chonbuk National University)
Yongseong Kim (Division of Chemistry and Chemical Engineering, Kyungnam University)
Publication Information
Journal of the Korean Chemical Society / v.47, no.2, 2003 , pp. 127-132 More about this Journal
Abstract
We have developed a direct polymerase chain reaction (PCR) and electrophoresis method for the diagnosis of bovine theileriosis from whole blood DNA analysis without DNA extraction. The technique empolyed a FoLT (formamide, low temp.) technique and was utilized in the diagnosis of bovine theileriosis. Formamide solubilize the blood cells, and the lowered incubation temperatures reduced protein coagulation. 100-200 nL of whole blood and PCR reagents were introduced directly into a PCR tube. After the amplification, the PCR product (816-bp DNA) was introduced into the electrophoresis system. The results of this analysis were consist with those obtained using purified DNA.
Keywords
T. buffeli(buffeli/orientalis/sergenti); Direct PCR; Whole Blood; Diagnosis;
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