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Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis  

Gang, Dae Cheon (Division of Chemical Engineering, Kyungnam University)
Jo, Seung Il (School of Electrical Engineering and Computer Science, Seoul National University)
Jeong, Du Su (School of Chemistry, Seoul National University)
Choe, Gyu Seong (Division of Chemical Engineering, Kyungnam University)
Kim, Yong Seong (Division of Chemical Engineering, Kyungnam University)
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Chiral separation of gemifloxacin, an quinolone antibacterial agent, using (+)-(18-crown-6)-tetracar-boxylic acid $(18C6H_4)$ as a chiral selector was performed by capillary electrophoresis (CE). Direct analysis of quinolone antibacterial agent in body fluid is beneficial in terms of fast analysis time, multicomponent analysis. However, high con-centration of sodium ion in body fluid can prevent gemifloxacin from interacting with $18C6H_4$ since sodium ion has high affinity with $18C6H_4$ due to the strong charge interaction. Ethylenediaminetetraacetic acid (EDTA), as a chelating ligand, was added in the running buffer in order to reduce the interaction between sodium ion and the chiral selector. Increased separation efficiency and reduced migration time were observed while sodium ion exists in the sample solution at the concentration up to 150 mM.
Capillary Elecrophoresis; Chiral Separation; Ethylenediaminetetraacetic Acid; (+)-(18-crown-6)-tetracar-boxylic Acid;
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1 Haginaka, J. J. Chromatogr. A. 2000, 875, 235.   DOI   ScienceOn
2 Schurig, V. J. Chromatogr. A.1994, 666, 111.   DOI   ScienceOn
3 Fanali, S. J. Chromatogr. A. 1997, 792, 227.   DOI   ScienceOn
4 Kuhn, R. Electrophoresis 1999, 20, 2605.   DOI   ScienceOn
5 A. E. Martell.; R. M. Smith. Critical Stability Constants; Plenum Press: New York., 1974, 1, 204.
6 Szejtii, J. Chem. Rev. 1998, 98, 1743.   DOI   ScienceOn
7 Otsuka, K.; Terabe, S. J. Chromatogr. A. 2000, 875, 163.   DOI   ScienceOn
8 Baker, D. R. Capillary Electrophoresis; John Wiley &Son: New York., 1995; p 44.
9 Stewart, C. J.; Iles, R. K.; Perrett, D. Electrophoresis.2001, 22, 1136.   DOI   ScienceOn
10 Seiler, H.; Sigel, A.; Sigel, H. Handbook on Metals inClinical and Analytical Chemistry; Marcel Dekker:New York., 1994; p 1.
11 Kuhn, R.; Wagner, J.; Walbroehl, Y.; Bereuter, T. Electrophoresis.1994, 15, 828.   DOI   ScienceOn
12 Jang, j.; Cho, S. I.; Jung, H.; Chung, D. S. Electrophoresis.2001, 22, 4362.   DOI   ScienceOn
13 Machida, Y.; Nishi, H.; Nakumura, K.; Nakai, H.; Sato,T. J. Chromatogr. A. 1998, 805, 85.   DOI   ScienceOn
14 Zhang, X. X.; Bradshaw, J. S.; Izatt, R. M. Chem. Rev.1997, 97, 3313.   DOI   ScienceOn
15 Vespalec, R.; Bocek, P. Chem. Rev. 2000, 700, 3715.
16 Hong, C. Y.; Kim, Y. K.; Chang, J. H.; Kim, Y. Z.;Kawk, J. H. J. Med. Chem. 1997, 40, 3584.   DOI   ScienceOn
17 Hyun, M. H.; Jin, J. S.; Lee, W. Bull. Korea Chem. Soc.1998, 19, 819.
18 Behr, J.-P.; Lehn, J.-M.; Vierling, P.; Helv. Chim. Acta.1982, 65, 1853.   DOI
19 Cho, S. I.; Jung, H.; Chung, D. S. Electrophoresis. 2000,21, 3618.   DOI   ScienceOn