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http://dx.doi.org/10.5012/jkcs.2002.46.5.412

Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis  

Gang, Dae Cheon (Division of Chemical Engineering, Kyungnam University)
Jo, Seung Il (School of Electrical Engineering and Computer Science, Seoul National University)
Jeong, Du Su (School of Chemistry, Seoul National University)
Choe, Gyu Seong (Division of Chemical Engineering, Kyungnam University)
Kim, Yong Seong (Division of Chemical Engineering, Kyungnam University)
Publication Information
Journal of the Korean Chemical Society / v.46, no.5, 2002 , pp. 412-429 More about this Journal
Abstract
Chiral separation of gemifloxacin, an quinolone antibacterial agent, using (+)-(18-crown-6)-tetracar-boxylic acid $(18C6H_4)$ as a chiral selector was performed by capillary electrophoresis (CE). Direct analysis of quinolone antibacterial agent in body fluid is beneficial in terms of fast analysis time, multicomponent analysis. However, high con-centration of sodium ion in body fluid can prevent gemifloxacin from interacting with $18C6H_4$ since sodium ion has high affinity with $18C6H_4$ due to the strong charge interaction. Ethylenediaminetetraacetic acid (EDTA), as a chelating ligand, was added in the running buffer in order to reduce the interaction between sodium ion and the chiral selector. Increased separation efficiency and reduced migration time were observed while sodium ion exists in the sample solution at the concentration up to 150 mM.
Keywords
Capillary Elecrophoresis; Chiral Separation; Ethylenediaminetetraacetic Acid; (+)-(18-crown-6)-tetracar-boxylic Acid;
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