Browse > Article
http://dx.doi.org/10.5012/bkcs.2014.35.9.2749

Graphene Oxide-based Direct Measurement of DNase I Activity with Single Stranded DNA  

Gang, Jongback (Department of Nano Chemistry, Gachon University)
Publication Information
Bulletin of the Korean Chemical Society / v.35, no.9, 2014 , pp. 2749-2752 More about this Journal
Abstract
Recent studies have shown that single-stranded DNA adsorbed onto graphene oxide is protected from DNase I cleavage. However, double-stranded DNA bound to graphene oxide and could be digested by DNase I. To elucidate whether single-stranded DNA is protect from DNase I in the presence of graphene oxide, this study conducted DNase I digestion using single-stranded DNA and single-stranded DNA containing the duplex region in the presence of graphene oxide. Addition of DNase I resulted in restoration of the fluorescence emission that had been quenched when DNA was adsorbed to graphene oxide. It indicates that DNase I cleaved the adsorbed single-stranded DNA onto graphene oxide, which was sufficient for the detection of DNase I activity.
Keywords
Graphene oxide; Platform; Biosensor; DNase I;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Wang, H.; Hu, Y. H. Ind. Eng. Chem. Res. 2011, 50, 6132.   DOI   ScienceOn
2 Wu, M.; Kempaiah, R.; Huang, P.-J. J.; Maheshiwari, V.; Liu, J. Langmuir 2011, 21, 2731.
3 Nelson, T.; Zhang, B.; Prezhdo, O. V. Nano Lett. 2010, 10, 3237.   DOI   ScienceOn
4 Lu, C.-H.; Yang, H.-H.; Zhu, C.-L.; Chen, X.; Chen, G.-N. Angew. Chem. 2009, 121, 4879.   DOI   ScienceOn
5 He, S. J.; Song, B.; Li, D.; Zhu, C. F.; Qi, W. P.; Wen, Y. Q.; Wang, L. H.; Song, S. P.; Fang, H. P.; Fan, C. Adv. Funct. Mater. 2010, 20, 453.   DOI   ScienceOn
6 Zhou, X.-H.; Ma, Q.-J.; Wu, X.-X.; Zhu, X. Analytica Chimica Acta 2012, 727, 67.   DOI   ScienceOn
7 Peng, L.; Zhu, Z.; Chen, Y.; Han, D.; Tan, W. Biosensors and Bioelectronics 2012, 35, 475.   DOI   ScienceOn
8 Lu, C.-H.; Li, J.; Liu, J.-J.; Yang, H.-H.; Chen, X.; Chen, G.-N. Chem. Eur. J. 2010, 16, 4889.   DOI   ScienceOn
9 Li, F.; Huang; Y.; Yang; Q.; Zhong; Z.; Li, D.; Wang, L. Nanoscale 2010, 2, 1021.   DOI   ScienceOn
10 Tang, Z.; Wu, H.; Cort, J. R.; Buchko, G. W.; Zhang, Y.; Shao, Y.; Aksay, I. A.; Liu, J.; Lin, Y. Small 2010, 6, 1205.   DOI   ScienceOn
11 Zhou, Z.; Zhu, C.; Ren, J.; Dong, S. Analytica Chimica Acta 2012, 740, 88.   DOI   ScienceOn
12 Galas, D. J.; Schmitz, A. Nucleic Acids Research 1978, 5, 3157.   DOI   ScienceOn
13 Campbell, V. W.; Jackson, D. A. J. Biol. Chem. 1980, 255, 3726.
14 Jeltsch, A.; Fritz, A.; Alves, J.; Wolfes, H.; Pingoud, A. Anal. Biochem. 1993, 213, 234.   DOI
15 Krieg, P. A.; Melton, D. A. Meth. Enzymol.1987, 155, 397.   DOI
16 Wang, P.; Li, Z.; Wang, J.; Li, J.; Lin, C. Trend in Biotechnology 2011, 29, 205.   DOI   ScienceOn
17 Geim, A. K.; Novoselov, K. S. Nature Materials 2007, 6, 183.   DOI   ScienceOn
18 Chen, H.; Muller, M.; Gilmore, K. J.; Wallace, G. G.; Li, D. Adv. Mater. 2008, 20, 3557.   DOI   ScienceOn
19 Zhou, M.; Zhai, Y. M.; Dong, S. J. Anal. Chem. 2009, 81, 5603.   DOI   ScienceOn
20 Wang, Y.; Li, Z. H.; Hu, D. H.; Lin, C. T.; Li, J. H.; Lin, Y. H. J. Am. Chem. Soc. 2010, 132, 9274.   DOI   ScienceOn
21 Lu, C.-H.; Zhu, C.-L.; Li, J.; Liu, J.-J.; Chen, X.; Yang, H.-H. Chem. Commun. 2010, 3116.
22 Jang, J. H.; Kim, Y. K.; Kwon. H. M.; Yeo, W. S.; Kim, D. E.; Min, D. H. Angew. Chem. Int. Ed. 2010, 49, 5703.   DOI   ScienceOn