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http://dx.doi.org/10.5012/bkcs.2014.35.9.2704

Liquid Crystal Droplet Patterns to Monitor Catalase Activity at Femtomolar Levels  

Yoon, Stephanie (Department of Chemistry, Gachon University)
Jang, Chang-Hyun (Department of Chemistry, Gachon University)
Publication Information
Bulletin of the Korean Chemical Society / v.35, no.9, 2014 , pp. 2704-2710 More about this Journal
Abstract
Catalase (CAT) decomposes hydrogen peroxide that is toxic to the body. In this study, simple and sensitive detector has been developed for observing catalase activity using liquid crystal droplet system. Microscale LC droplet patterns are formed by spreading aldehyde-doped nematic liquid crystal on pre-treated glass slides. When hydrogen peroxide is added, aldehyde is oxidized and amphiphiles are formed. Dodecanoates cause the pattern to transit from bright to dark as they self-assemble to form a carboxyalte monolayer at the interface. When a drop of pre-incubated CAT and hydrogen peroxide mixture is placed onto the pattern, bright fan-shape is observed. This planar optical appearance indicates that catalase has decomposed hydrogen peroxide. Compared to the detectors that have been previously developed, this system is more sensitive with detection limit of 1fM. This research suggests further studies to be on LC droplet patterning to develop highly sensitive and methodologically simple sensors for various chemicals.
Keywords
Liquid crystal; 4-Cyano-4'-pentylbiphenyl (5CB); Droplet pattern; Catalase; Hydrogen peroxide;
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