[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5012/bkcs.2014.35.4.1087

Genetic Incorporation of a Phenanthroline-Containing Amino Acid in Escherichia coli

Jin, Sunhwa (Department of Chemistry, Sogang University)
Lee, Hui-Jung (Department of Chemistry, Sogang University)
Lee, Sangyeul (Department of Chemistry, Sogang University)
Lee, Hyun Soo (Department of Chemistry, Sogang University)

Publication Information

Bulletin of the Korean Chemical Society / v.35, no.4, 2014 , pp. 1087-1090 More about this Journal

Abstract

A simple and general method that selectively introduces metal binding sites into a protein can greatly increase the ability to design and biosynthesize artificial metalloproteins. Here, we report the incorporation of a phenanthroline-containing amino acid (Phen-Ala) into proteins in Escherichia coli by using the $tRNA{^{Tyr}}_{CUA}$ and tyrosyl aminoacyl-tRNA synthetase pair (BpyRS) from Methanococcus jannaschii, which was originally developed for a bipyridine-containing amino acid (Bpy-Ala). The incorporation efficiency of BpyRS for Phen-Ala was comparable to that for Bpy-Ala. Because of its high metal-binding ability and characteristic spectral properties, Phen-Ala can be a useful alternative to the existing metal-chelating amino acids for the design and synthesis of artificial metalloproteins.

Keywords

Phenanthroline; Metal-chelating amino acids; Proteins;

Citations & Related Records

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