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http://dx.doi.org/10.5012/bkcs.2014.35.4.1036

PAMAM Dendrimer Conjugated with N-terminal Oligopeptides of Mouse Fibroblast Growth Factor 3 as a Novel Gene Carrier  

Jung, Jinwoo (Graduate School and Department of Analytical Science and Technology (GRAST), Chungnam Nationl University)
Lee, Jeil (Graduate School and Department of Analytical Science and Technology (GRAST), Chungnam Nationl University)
Kim, Tae-Hun (Department of Biochemistry, College of Natural Sciences, Chungnam National University)
Yang, Bong Suk (Graduate School and Department of Analytical Science and Technology (GRAST), Chungnam Nationl University)
Lee, Eunji (Graduate School and Department of Analytical Science and Technology (GRAST), Chungnam Nationl University)
Kim, Youn-Joong (Korea Basic Science Institute (KBSI))
Park, Jong-Sang (School of Chemistry and Molecular Engineering, Seoul National University)
Choi, Joon Sig (Graduate School and Department of Analytical Science and Technology (GRAST), Chungnam Nationl University)
Publication Information
Bulletin of the Korean Chemical Society / v.35, no.4, 2014 , pp. 1036-1042 More about this Journal
Abstract
In this study, we introduced the RRLR oligopeptide sequences on the surface of polyamidoamine (PAMAM) dendrimer and characterized the physical properties and gene carrier activity of the novel polymer using HEK 293, NIH3T3, and HeLa cells. The RRLR peptide sequences were derived from a mouse fibroblast growth factor 3 (FGF3) protein containing a bipartite NLS motif. The entire sequence of FGF3 is RLRRDAGGRGGVYEHLGGAPRRRK and it has two functional sequences RLRR and RRRK at N-terminus and C-terminus, respectively. In particular, PAMAM G4-RRLR conferred enhanced transfection efficiency and lower cytotoxicity compared with those of PEI 25 kDa, PAMAM G4-R, and PAMAM G4 in various cell lines. These results suggest that the introduction of N-terminal oligopeptides of FGF3 on the surface of PAMAM holds promise as an effective non-viral gene delivery carrier for gene therapy.
Keywords
Gene delivery; PAMAM dendrimer; Peptide; Mouse fibroblast growth factor 3;
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