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http://dx.doi.org/10.5012/bkcs.2013.34.6.1839

Synthesis and Evaluation of Molecularly Imprinted Polymeric Microspheres for Chloramphenicol by Aqueous Suspension Polymerization as a High Performance Liquid Chromatography Stationary Phase  

Zhang, Yan (School of Civil and Environment Engineering, University of Science and Technology Beijing)
Lei, Jiandu (Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University)
Publication Information
Bulletin of the Korean Chemical Society / v.34, no.6, 2013 , pp. 1839-1844 More about this Journal
Abstract
Molecularly imprinted microsphere for chloramphenicol (CAP) with high adsorption capacity and excellent selectivity is prepared by aqueous suspension polymerization, in which chloramphenicol is used as template molecule and ethyl acetate as porogen. The CAP-imprinted microspheres are used as high performance liquid chromatography (HPLC) stationary phase and packed into stainless steel column ($150mm{\times}4.6mm$ i.d.) for selective separation of chloramphenicol. HPLC analysis suggests that chloramphenicol can be distinguished from not only its structural analogs but also other broad-spectrum antibiotic such as erythromycin and tetracycline. In addition, the binding experiments of CAP-imprinted microspheres are carried out in ethanol/water (1:4, V:V), the results indicate that the maximum apparent static binding capacity of molecularly imprinted microspheres is up to 66.64 mg $g^{-1}$ according to scatchard model.
Keywords
Molecularly imprinted polymer; Microsphere; Chloramphenicol; Stationary phase;
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1 Shi, X. Z.; Wu, A. B.; Zheng, S. L.; Li, R. X.; Zhang, D. B. J. Chromatogr. B 2007, 850, 24.   DOI   ScienceOn
2 Guo, L. Y.; Guan, M.; Zhao, C. D.; Zhang, H. X. Anal. Bioanal. Chem. 2008, 392, 1431.   DOI
3 Schirmer, C.; Meisel, H. Anal. Bioanal. Chem. 2008, 392, 223.   DOI
4 Schirmer, C.; Meisel, H. Anal. Bioanal. Chem. 2009, 394, 2249.   DOI
5 Chaitidou, S.; Kotrotsiou, O.; Kotti, K.; Kammona, O.; Bukhari, M.; Kiparissides, C. Mater. Sci. Eng. B 2008, 152, 55.   DOI   ScienceOn
6 Lei, J. D.; Tan, T. W. Biochem. Eng. J. 2002, 11, 175.   DOI   ScienceOn
7 Scathard, G.; Scheiberg, I. I.; Armstrong, S. H. J. Am. Chem. Soc. 1950, 72, 535.   DOI
8 Woodward, K. N. In Watson, D. H., Ed.; Pesticide, Veterinary and Other Residues in Food; Woodward Publisher Limited: Cambridge, 2004; p 176, Chapter 8.
9 Commission Decision 2003/181/EC of 13 March 2003 amending decision 2002/657/EC as regard the setting of minimum required performance limits (MRPLs) for certain residues in food of animal origin, Off. J. Eur. Commun. 2003, L71, p 17.
10 Ferguson, J.; Young, A. baxter. P.; Kennedy, G.; Elliott, C.; Weigel, S.; Gatermann, R.; Ashwin, H.; Stead, S.; Sharman, M. Anal. Chim. Acta 2005, 529, 109.   DOI   ScienceOn
11 Impens, S.; Reybroeck, W.; Vercammen, J.; Courtheyn, D.; Ooghe, S.; De Wasch, K.; Smedts, W.; De Brabander, H. Anal. Chim. Acta 2003, 483, 153.   DOI   ScienceOn
12 Posyniak, A.; Zmudzki, J.; Niedzielska, J. Anal. Chim. Acta 2003, 483, 307.   DOI   ScienceOn
13 Shen, H. Y.; Jiang, H. L. Anal. Chim. Acta 2005, 535, 33.   DOI   ScienceOn
14 Kubala-Drincic, H.; Bazulic, D.; Sapunar-postruznik, J.; Grubelic, M.; Stuhne, G. J. Agric. Food. Chem. 2003, 51, 871.   DOI   ScienceOn
15 Mottier, P.; Parisod, V.; Gremaud, E.; Guy, P. A.; Stadler, R. H. J. Chromatogr. A 2003, 994, 75.   DOI   ScienceOn
16 Ronning, H. T.; Einarsen, K.; Asp, T. N. J. Chromatogr. A 2006, 1118, 226.   DOI   ScienceOn
17 Boyd, B.; Bjork, H.; Billing, J.; Shimelis, O.; Axelsson, S.; Leonora, M.; Yilmaz, E. J. Chromatogr. A 2007, 1174, 63.   DOI   ScienceOn
18 Huynh, T. P.; Pietrzyk-Le, A.; Bikram, K. C. C.; Noworyta, K. R.; Sobczak, J. W.; Sharma, P. S.; D'Souza, F.; Kutner, W. Biosen. Bioelectron. 2013, 41, 634.   DOI   ScienceOn
19 Norrlow, O.; Glad, M.; Mosbach, K. J. Chromatogr. A 1984, 299, 29.   DOI   ScienceOn
20 Hoshino, Y.; Kodama, T.; Okahata, Y.; Shea, K. J. J. Am. Chem. Soc. 2008, 130, 15242.   DOI   ScienceOn
21 Zhou, C. H.; Wang, T. T.; Liu, J. Q.; Guo, C.; Peng, Y.; Bai, J. L.; Liu, M.; Dong, J. W.; Gao, N.; Ning, B. A.; Gao, Z. X. Analyst. 2012, 137, 4469.   DOI   ScienceOn
22 Schirmer, C.; Meisel, H. J. Chromatogr. A 2006, 1132, 325.   DOI   ScienceOn
23 Wang, D. X.; Hong, S. P.; Row, K. H. Korean J. Chem. Eng. 2004, 21, 853.   DOI   ScienceOn
24 Suarez-Rodriguez, J. L.; Diaz-Garcia, M. E. Biosens. Bioelectr. 2001, 16, 955.   DOI   ScienceOn