Browse > Article
http://dx.doi.org/10.5012/bkcs.2013.34.4.1120

High-yield Expression and Characterization of Syndecan-4 Extracellular, Transmembrane and Cytoplasmic Domains  

Choi, Sung-Sub (Department of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Kim, Ji-Sun (Department of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Song, Jooyoung (Department of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Kim, Yongae (Department of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Publication Information
Bulletin of the Korean Chemical Society / v.34, no.4, 2013 , pp. 1120-1126 More about this Journal
Abstract
The syndecan family consists of four transmembrane heparan sulfate proteoglycans present in most cell types and each syndecan shares a common structure containing a heparan sulfate modified extracellular domain, a single transmembrane domain and a C-terminal cytoplasmic domain. To get a better understanding of the mechanism and function of syndecan-4 which is one of the syndecan family, it is crucial to investigate its three-dimensional structure. Unfortunately, it is difficult to prepare the peptide because it is membrane-bound protein that transverses the lipid bilayer of the cell membrane. Here, we optimize the expression, purification, and characterization of transmembrane, cytoplasmic and short extracellular domains of syndecan4 (syndecan-4 eTC). Syndecan-4 eTC was successfully obtained with high purity and yield from the M9 medium. The structural information of syndecan-4 eTC was investigated by MALDI-TOF mass (MS) spectrometry, circular dichroism (CD) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. It was confirmed that syndecan-4 eTC had an ${\alpha}$-helical multimeric structure like transmembrane domain of syndecan-4 (syndecan-4 TM) in membrane environments.
Keywords
Syndecan; Syndecan-4; Transmembrane; NMR spectroscopy; Membrane protein;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Woods, A.; Couchman, J. R. Curr. Opin. Cell Biol. 2001, 13, 578.   DOI   ScienceOn
2 Zhang, Y.; Pasparakis, M.; Kollias, G.; Simons, M. J. Biol. Chem. 1999, 274, 14786.   DOI   ScienceOn
3 Baba, F.; Swartz, K.; Buren, R.; Eickhoff, J.; Zhang, Y.; Wolberg, W.; Friedl, A. Breast Cancer Res. Tr. 2006, 98, 91.   DOI   ScienceOn
4 Lendorf, M. E.; Manon-Jensen, T.; Kronqvist, P.; Multhaupt, H. A. B.; Couchman, J. R. J. Histochem. Cytochem. 2011, 59, 615.   DOI   ScienceOn
5 Alexander, C. M.; Reichsman, F.; Hinkes, M. T.; Lincecum, J.; Becker, K. A.; Cumberledge, S.; Bernfield, M. Nat. Genet. 2000, 25, 329.   DOI   ScienceOn
6 Huang, W.; Chiquet-Ehrismann, R.; Moyano, J. V.; Garcia-Pardo, A.; Orend, G. Cancer Res. 2001, 161, 8586.
7 Chipquet-Ehrismann, R.; Kalla, P.; Pearson, C. A.; Beck, K.; Chiquet, M. Cell 1998, 53, 383.
8 Longley, R. L.; Woods, A.; Fleetwood, A.; Cowling, G. J.; Gallagher, J. T.; Couchman, J. R. J. Cell. Sci. 1999, 112, 3421.
9 Okina, E.; Manon-Jensen, T.; Whiteford, J. R.; Couchman, J. R. Scand. J. Med. Sci. Sports 2009, 19, 479.   DOI   ScienceOn
10 Horowitz, A.; Tkachenko, E.; Simons, M. J. Cell Sci. 2002, 157, 715.   DOI   ScienceOn
11 Couchman, J. R.; Vogt, S.; Lim, S. T.; Oh, E. S.; Prestwich, G. D.; Theibert, A.; Lee, W.; Woods, A. J. Biol. Chem. 2002, 277, 49296.   DOI   ScienceOn
12 Song, Y.; McFarland, D. C.; Velleman, S. G. Comp. Biochem. Physiol. Pt. A 2012, 161, 271.   DOI   ScienceOn
13 Oh, E. S.; Woods, A.; Couchman, J. R. J. Biol. Chem. 1997, 272, 11805.   DOI   ScienceOn
14 Lee, D.; Oh, E. S.; Woods, A.; Couchman, J. R.; Lee, W. J. Biol. Chem. 1998, 273, 13022.   DOI   ScienceOn
15 Whiteford, J. R.; Ko, S.; Lee, W.; Couchman, J. R. J. Biol. Chem. 2008, 283, 29322.   DOI   ScienceOn
16 Park, T. J.; Kim, J. S.; Choi, S. S.; Kim, Y. Protein Expr. Purif. 2009, 65, 23.   DOI   ScienceOn
17 Opella, S. J. Nat. Struct. Biol. 1997, 4, 845.
18 Park, T. J.; Kim, J. S.; Ahn, H. C.; Kim, Y. Biophys. J. 2011, 101, 1193.   DOI   ScienceOn
19 Opella, S. J.; Marassi, F. M. Chem. Rev. 2004, 104, 3587.   DOI   ScienceOn
20 Park, T. J.; Lee, M. H.; Kim, J. S.; Kim, Y. Process Biochem. 2011, 46, 1166.   DOI   ScienceOn
21 Walkenhorst, W. F.; Merzlyakov, M.; Hristova, K.; Wimley, W. C. Biochim. Biophs. Acta 2009, 1788, 1321.   DOI   ScienceOn
22 Grefrath, S. P.; Reynolds, J. A. Proc. Nat. Acad. Sci. USA 1974, 71, 3913.   DOI   ScienceOn
23 Rath, A.; Glibowicka, M.; Nadeau, V. G.; Chen, G.; C. Deber, M. PNAS 2008, 106, 1760.
24 Shin, J.; Song, Y.; McFarland, D. C.; Velleman, S. G. Mol. Cell Biochem. 2012, 363, 437.   DOI
25 Elenius, K.; Kalkanen, M. J. Cell Sci. 1994, 107, 2975.
26 Choi, Y.; Kim, S.; Lee, J.; Ko, S. G.; Lee, W.; Han, I. O.; Woods, A.; Oh, E. S. Eur. J. Cell Biol. 2008, 87, 807.   DOI   ScienceOn
27 Gopal, S.; Bober, A.; Whiteford, J. R.; Multhaupt, H. A. B.; Yoneda, A.; Couchman, J. R. J. Biol. Chem. 2010, 19, 14247.
28 Dews, I. C.; MacKenzie, K. R. PNAS 2007, 104, 20782.   DOI   ScienceOn
29 Häcker, U.; Nybakken, K.; Perrimon, N. Nat. Rev. Mol. Cell Bio. 2005, 6, 530.   DOI   ScienceOn
30 David, G.; Schueren, B.; Marynen, P.; Cassiman, J. J.; Berhe, H. J. Cell Biol. 1992, 11, 961.
31 Tetsuhito, K.; Shworak, N. W.; Rosenberg, R. D. J. Biol. Chem. 1992, 287, 4870.
32 Alexopoulou, A. N.; Multhaupt, H. A. B.; Couchman, J. R. Int. J. Biochem. Cell 2007, 39, 505.   DOI   ScienceOn
33 Beauvais, D. M.; Rapraeger, A. C. Reprod. Biol. Endocrin. 2004, 2, 3.   DOI   ScienceOn
34 Gulyás, M.; Hjerper, A. J. Pathol. 2003, 199, 479.   DOI   ScienceOn
35 Roskams, T.; De Vos, R.; David, G.; Damme, B.; Desmet, V. J. Pathol. 1998, 185, 290.   DOI
36 Echtermeyer, F.; Streit, M.; Wilcox-Adelman, S.; Saoncella, S.; Denhez, F.; Detmar, M.; Goetinck, P. F. J. Clin. Invest. 2001, 107, R9.   DOI   ScienceOn
37 Shimazu, A.; Bachchu, M. A. H.; Morishita, M.; Noshiro, M.; Kato, Y.; Iwamoto, Y. J. Dent. Res. 1999, 78, 1791.   DOI   ScienceOn
38 Yung, S.; Woods, A.; Chan, T. M.; Davies, M.; Williams, J. D.; Couchman, J. R. FASEB J. 2001, 15, 1631.
39 Hirabayashi, K.; Numa, F.; Suminami, Y.; Murakami, A.; Murakami, T.; Kato, H. Tumour Biol. 1998, 19, 454.   DOI   ScienceOn