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http://dx.doi.org/10.5012/bkcs.2013.34.3.922

Detection of PCB77 by Indirect Competitive Enzyme-linked Immunosorbent Assay in Sea Sediment Samples  

Chen, Han-Yu (School of Environmental Science and Engineering, Shanghai Jiao Tong University)
Zhuang, Hui-Sheng (School of Environmental Science and Engineering, Shanghai Jiao Tong University)
Yang, Guang-Xin (School of Environmental Science and Engineering, Shanghai Jiao Tong University)
Publication Information
Bulletin of the Korean Chemical Society / v.34, no.3, 2013 , pp. 922-926 More about this Journal
Abstract
3,3',4,4'-Tetrachlorobiphenyl (IUPAC PCB77) is one of seven indicative polychlorinated biphenyls (PCBs) in the surface sediments. The current study presents a novel polyclonal antibody for the determination of the PCB77 using indirect competitive enzyme-linked immunosorbent assay. Under optimum conditions, PCB77 was determined within the concentration range of 0.01-100 ${\mu}g\;L^{-1}$, with a detection limit of 0.057 ${\mu}g\;L^{-1}$. The assays were tested for their cross-reactivity profiles using 3 selected congeners and 4 Aroclor products. The assays were highly specific for coplanar PCB congeners, but less specific for Aroclor1248. The spiked recoveries from five sediment samples were 86%-114% for PCB77 from ELISA, which were satisfactory. The current study demonstrated that the developed antiserum and immunoassay procedure can be used to detect PCB77 in environmental samples. The results of the sediment analysis were confirmed by conventional GC/ECD.
Keywords
3.3',4.4'-Tetrachlorobiphenyl (IUPAC PCB77); Immunoassays; Coplanar polychlorinated biphenyls; Antigen; Polyclonal antibody;
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