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http://dx.doi.org/10.5012/bkcs.2013.34.3.823

Low-ε Static Probe Development for 15N-1H Solid-state NMR Study of Membrane Proteins for an 800 MHz NB Magnet  

Park, Tae-Joon (Department of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Choi, Sung-Sub (Department of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Jung, Ji-Ho (Department of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Park, Yu-Geun (Department of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Kim, Yongae (Department of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Publication Information
Bulletin of the Korean Chemical Society / v.34, no.3, 2013 , pp. 823-826 More about this Journal
Abstract
A low-${\varepsilon}$ solid-state NMR(Nuclear Magnetic Resonance) probe was developed for the spectroscopic analysis of two-dimensional $^{15}N-^1H$ heteronuclear dipolar coupling in dilute membrane proteins oriented in hydrated and dielectrically lossy lipid environments. The system employed a 800 MHz narrow-bore magnet. A solenoid coil strip shield was used to reduce deleterious RF sample heating by minimizing the conservative electric fields generated by the double-tuned resonator at high magnetic fields. The probe's design, construction, and performance in solid-state NMR experiments at high magnetic fields are described here. Such high-resolution solid-state NMR spectroscopic analysis of static oriented samples in hydrated phospholipid bilayers or bicelles could aid the structural analysis of dilute biological membrane proteins.
Keywords
Solid-state NMR probe; Low-${\varepsilon}$; Probe design; Membrane proteins; Bicelles;
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