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http://dx.doi.org/10.5012/bkcs.2013.34.2.524

Quantitative Analysis of Growth of Cells on Physicochemically Modified Surfaces  

Chandra, Prakash (Department of Chemistry, Kongju National University)
Kim, Jihee (Department of Chemistry, Kongju National University)
Rhee, Seog Woo (Department of Chemistry, Kongju National University)
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Abstract
In this study, we describe the most expected behavior of cells on the modified surface and the correlation between the modified substrates and the response of cells. The physicochemical characteristics of substrates played an essential role in the adhesion and proliferation of cells. Glass and polymer substrates were modified using air plasma oxidation, and the surfaces were coated with self-assembled monolayer molecules of silanes. The PDMS substrates embedded with parallel micropatterns were used for evaluation of the effect of topologically modified substrate on cellular behaviour. BALB/3T3 fibroblast cells were cultured on different surfaces with distinct wettability and topology, and the growth rates and morphological change of cells were analyzed. Finally, we found the optimum conditions for the adhesion and proliferation of cells on the modified surface. This study will provide insight into the cell-surface interaction and contribute to tissue engineering applications.
Keywords
Cell-surface interaction; Surface modification; Contact angle; Growth curve;
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1 LeBaron, R. G.; Athanasiou, K. A. Tissue Eng. 2000, 6, 85.   DOI   ScienceOn
2 Ratner B. D.; Hoffman, A. S.; Schoen, F. J.; Lemons, J, In Biomaterials Science: An Introduction to Materials in Medicine; 2nd Edition, Elsevier, California, 2004.
3 Ruoslahti, E. Annu. Rev. Cell Dev. Biol. 1996, 12, 697.   DOI   ScienceOn
4 Boateng, S. Y.; Lateef, S. S.; Mosley, W.; Hartman, T. J.; Hanley, L.; Russell, B. Am. J. Physiol. Cell. Physiol. 2005, 288, C30.
5 van Wachem, P. B.; Beugeling, T.; Feijen, J.; Bantjes, A.; Detmers, J. P.; van Aken, W.G. Biomaterials 1985, 6, 403.   DOI   ScienceOn
6 van Wachem, P. B.; Hogt, A. H.; Beugeling, T.; Feijen, J.; Bantjes, A.; Detmers, J. P.; van Aken, W. G. Biomaterials 1987, 8, 323.   DOI   ScienceOn
7 Arima, Y.; Iwata, H. Biomaterials 2007, 28, 3074.   DOI   ScienceOn
8 Balcells, M.; Edelman, E. R. J. Cell. Physiol. 2002, 191, 155.
9 van Kooten, T. G.; Spijker, H. T.; Busscher, H. J. Biomaterials 2004, 25, 1735.   DOI   ScienceOn
10 Ponsonnet, L.; Reybier, K.; Jaffrezic, N.; Comte, V.; Lagneau, C.; Lissac, M.; Martelet, C. Mater. Sci. Eng. C 2003, 23, 551.   DOI   ScienceOn
11 Ponche, A.; Bigerelle, M.; Anselme, K. Proc. IMechE, Part H: J. Eng. Med. 2010, 224, 1471.   DOI   ScienceOn
12 Anselme, K.; Ponche, A.; Bigerelle, M.; Anselme, K. Proc. IMechE, Part H: J. Eng. Med. 2010, 224, 1487.
13 Refai, A. K.; Textor, M.; Brunette, D. M.; Waterfield, J. D. J. Biomed. Mater. Res. 2004, 70A, 194.   DOI
14 Andrade, J. D. In Surface and Interfacial Aspects of Biomedical Polymers: Protein Adsorption; Andrade, J. D. Ed.; Plenum Press: New York, 1985, Vol 2; p1.
15 Horbett, T. A.; Brash, J. L. In Proteins at Interfaces: Physiochemical and Biochemical Studies; Brash, J. L.; Horbett, T. A. Ed.; ACS Symposium Series 343, Washington, D.C., 1987; p 1.
16 Miyamoto, A.; Teramoto, H.; Coso, O. A.; Gutkind, J. S.; Burbelo, P. D.; Akiyama, S. K.; Yamada, K. M. J. Cell Biol. 1995, 131, 791.   DOI   ScienceOn
17 Streuli, C. H. J. Cell Sci. 2009, 122, 171.   DOI   ScienceOn
18 Lee, J. H.; Lee, J. W.; Khang, G.; Lee, H. B. Biomaterials 1997, 18, 351.   DOI   ScienceOn
19 Lee, J. H.; Khang, G.; Lee, J. W.; Lee, H. B. J. Colloid Interf. Sci. 1998, 205, 323.   DOI   ScienceOn
20 Baxter, L. C.; Frauchiger, V.; Textor, M.; ap Gwynn, I.; Richards, R. G. Eur. Cell. Mater. 2002, 4, 1.
21 Mahto, S. K.; Yoon, T. H.; Shin, H.; Rhee, S. W. Biomed. Microdevices 2009, 11, 401.   DOI   ScienceOn
22 Rhee, S. W.; Taylor, A. M.; Tu, C. H.; Cribbs, D. H.; Cotman, C. W.; Jeon, N. L. Lab Chip 2005, 5, 102.   DOI   ScienceOn
23 Harnett, E. M.; Alderman, J.; Wood, T. Colloids Surf. B: Biointerfaces, 2007, 55, 90.   DOI   ScienceOn
24 Rebiscoul, D.; Perrut, V.; Renault, O.; Rieutord, F.; Olivier, S.; Haumesser, P.-H. J. Supercritical Fluids, 2009, 51, 287.   DOI   ScienceOn
25 Manifar, T.; Rezaee, A.; Sheikhzadeh, M.; Mittler, S. Appl. Surf. Sci. 2008, 254, 4611.   DOI   ScienceOn
26 Xue, C.-Y.; Chin, S. Y.; Khan, S. A.; Yang, K.-L. Langmuir 2010, 26, 3739.   DOI   ScienceOn
27 Eddington, D. T.; Puccinelli, J. P.; Beebe, D. J. Sens. Actuators B 2006, 114, 170.   DOI   ScienceOn
28 Gerecht, S.; Bettinger, C. J.; Zhang, Z.; Borenstein, J.; Vunjak- Novakovic, G.; Langer, R. Biomaterials 2007, 28, 4068.   DOI   ScienceOn
29 Hillborgl, H.; Gedde, U. W. IEEE Trans. on Dielectrics and Electrical Insulation, 1999, 6, 703.   DOI   ScienceOn
30 Wood, A. J. Cell Sci. 1988, 90, 667.
31 Dalby, M. J. Int. J. Nanomedicine 2007, 2, 373.
32 Dalby, M. J. Med. Eng. Phys. 2005, 27, 730.   DOI   ScienceOn
33 Liao, L.; Jaken, S. Cell Growth Diff. 1993, 4, 309.
34 Lee, K.; Song, K. Cell Cycle 2007, 6, 1487.
35 Walboomers, X. F.; Croes, H. J. E.; Ginsel, L. A.; Jansen, J. A. Biomaterials 1998, 19, 1861.   DOI   ScienceOn
36 den Braber, E. T.; de Ruijter, J. E.; Ginsel, L. A.; von Recum, A. F.; Jansen, J. A. Biomaterials 1996, 17, 2037.   DOI   ScienceOn
37 Wang, J. H.-C.; Grood, E. S.; Florer, J.; Wenstrup, R. J. Biomech. 2000, 33, 729.   DOI   ScienceOn