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http://dx.doi.org/10.5012/bkcs.2012.33.8.2592

Synthesis of Cysteine Capped Silver Nanoparticles by Electrochemically Active Biofilm and their Antibacterial Activities  

Khan, Mohammad Mansoob (School of Chemical Engineering, Yeungnam University)
Kalathil, Shafeer (School of Chemical Engineering, Yeungnam University)
Lee, Jin-Tae (School of Chemical Engineering, Yeungnam University)
Cho, Moo-Hwan (School of Chemical Engineering, Yeungnam University)
Publication Information
Abstract
Cysteine capped silver nanoparticles (Cys-AgNPs) have been synthesized by employing electrochemically active biofilm (EAB), $AgNO_3$ as precursor and sodium acetate as electron donor in aqueous solution at $30^{\circ}C$. Cys-AgNPs of 5-10 nm were synthesized and characterized by UV-Vis, FT-IR, XRD and TEM. Capping of the silver nanoparticles with cysteine provides stability to nanoparticles by a thiolate bond between the amino acid and the nanoparticle surface and hydrogen bonding among the Cys-AgNPs. In addition, the antibacterial effects of as-synthesized Cys-AgNPs have been tested against two pathogenic bacteria Escherichia coli (O157:H7) and Pseudomonas aeruginosa (PAO1). The results demonstrate that the as-synthesized Cys-AgNPs can proficiently inhibit the growth and multiplication of E. coli and P. aeruginosa.
Keywords
Cys-AgNPs; Silver colloidal nanoparticles; (L)-Cysteine; Electrochemically active biofilm(EAB); Hydrogen bonding;
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