[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5012/bkcs.2012.33.3.987

Sequence Coverage Enhancement Using Magnetic Nanoparticles in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Protein Analysis

Park, Eun-Hye (Department of Chemistry, Seoul National University)
Song, Jin-Su (Department of Chemistry, Seoul National University)
Kim, Hie-Joon (Department of Chemistry, Seoul National University)

Publication Information

Bulletin of the Korean Chemical Society / v.33, no.3, 2012 , pp. 987-992 More about this Journal

Abstract

Magnetic nanoparticles (MNPs) treated with phosphoric acid were used to improve sequence coverage in protein identification by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Sample solution of tryptic peptides from proteins was mixed with the MNPs, and the MNPs were separated from the supernatant using a magnet. MALDI mass spectra obtained separately from the supernatant and the MNPs were distinctly different and complementary to each other. Combination of the two spectra led to a significantly increased sequence coverage.

Keywords

Matrix-assisted laser desorption/ionization (MALDI); Sequence coverage; Magnetic nanoparticles (MNPs); Phosphoric acid;

Citations & Related Records

Times Cited By Web Of Science : 1 (Related Records In Web of Science)

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