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http://dx.doi.org/10.5012/bkcs.2012.33.2.571

Preparation of CdSe QDs-carbohydrate Conjugation and its Application for HepG2 Cells Labeling  

Jiang, Mingxing (School of Life Science, Anhui University)
Chen, Yan (School of Life Science, Anhui University)
Kai, Guiqing (School of Life Science, Anhui University)
Wang, Ruijun (School of Life Science, Anhui University)
Cui, Huali (School of Life Science, Anhui University)
Hu, Meili (School of Life Science, Anhui University)
Publication Information
Bulletin of the Korean Chemical Society / v.33, no.2, 2012 , pp. 571-574 More about this Journal
Abstract
In present study, CdSe quantum dots (QDs) were prepared with a novel but simple, effective and exercisable method. Nine different types of carbohydrate molecules were used to modify CdSe QDs. D-mannose (Man)-coated quantum dots were prepared for labeling human hepatoma (HepG2) cells, because of the high expression of mannose receptor (MR) on HepG2 cells. The uptake characteristics of CdSe QDs-Man were investigated in HepG2 cells. The absorption rate result of MTT assay in 48 h suggested the extremely low cytotoxicity of CdSe QDs-Man. The presence of quantum dots was confirmed with fluorescence microscopy. These results were encouraging regarding the application of QDs molecules for early detection of HepG2 cells.
Keywords
CdSe quantum dots; Carbohydrates molecules; HepG2 mannose receptor; Receptor-mediated endocytosis;
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