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http://dx.doi.org/10.5012/bkcs.2012.33.1.100

Spectroscopic and Electrochemical Detection of Thrombin/5'-SH or 3'-SH Aptamer Immobilized on (porous) Gold Substrates  

Park, Buem-Jin (Department of Chemical Engineering, Kwangwoon University)
Sa, Young-Seung (Department of Chemical Engineering, Kwangwoon University)
Kim, Yong-Hwan (Department of Chemical Engineering, Kwangwoon University)
Kim, Young-Hun (Department of Chemical Engineering, Kwangwoon University)
Publication Information
Bulletin of the Korean Chemical Society / v.33, no.1, 2012 , pp. 100-104 More about this Journal
Abstract
Thrombin is a serine protease that catalyzes the conversion of soluble fibrinogen to insoluble fibrin, and thus induces physiological and pathological blood coagulation. Therefore, it is important to detect thrombin in blood serum for purposes of diagnosis. To achieve this goal, it has been suggested that a 15-mer aptamer strongly binds with thrombin to form a G-quartet structure of the aptamer. Generally, 5'-end thiol-functionalized aptamer has been used as an anti-thrombin binder. Herein, we evaluate the possibility of utilizing a 3'-SH aptasensor for thrombin detection using SPR spectroscopy, and compare the enhancement of the electrochemical signal of the thrombin-aptamer bound on a porous gold substrate. Although the two aptamers have similar configurations, in SPR analysis, the 3'-SH aptamer was a effective aptasensor as well as 5'-SH aptamer. Results from electrochemical analysis showed that the porous gold substrate acted as a good substrate for an aptasensor and demonstrated 5-fold enhancement of current change, as compared to gold thin film.
Keywords
Aptamer; Thrombin; SPR sensor; CV; porous gold;
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1 Wang, Y.; He, X.; Wang, K.; Ni, X.; Su, J.; Chen, Z. Biosens. Bioelectron. 2011, 26, 3536.   DOI   ScienceOn
2 Bock, L. C.; Griffin, L. C.; Latham, J. A.; Vermaas, E. H.; Toole, J. J. Nature 1992, 355, 564.   DOI   ScienceOn
3 Smirnov, I.; Shafer, R. H. Biochemistry 2000, 39, 1462.   DOI   ScienceOn
4 Paborsky, L. R.; McCurdy, S. N.; Griffin, L. C.; Toole, J. J.; Leung, L. L. J. Biol. Chem. 1993, 268, 20808.
5 Tasset, D. M.; Kubik, M. F.; Steiner, W. J. Mol. Biol. 1997, 272, 688.   DOI   ScienceOn
6 Huang, D.-W.; Niu, C.-G.; Qin, P.-Z.; Ruan, M.; Zeng, G.-M. Talanta 2010, 83, 185.   DOI   ScienceOn
7 So, H.-M.; Won, K.; Kim, Y. H.; Kim, B.-K.; Ryu, B. H.; Na, P. S.; Kim, H.; Lee, J.-O. J. Am. Chem. Soc. 2005, 127, 11906.   DOI   ScienceOn
8 Cai, H.; Lee, T. M.-H.; Hsing, I.-M. Sens. Actuator B 2006, 114, 433.   DOI   ScienceOn
9 Zheng, Y.; Wang, Y.; Yang, X. Sens. Actuator B 2011, 156, 95.   DOI   ScienceOn
10 Jeong, S. H.; Kim, C. S.; Yang, J. BioChip J. 2010, 4, 141.   DOI   ScienceOn
11 Lee, J.-O.; So, H.-M.; Jeon, E.-K.; Chang, H.; Won, K.; Kim, Y. H. Anal. Bioanal. Chem. 2008, 390, 1023.   DOI   ScienceOn
12 Kim, Y. H.; Kim, J. P.; Han, S. J.; Sim, S. J. Sens. Actuator B 2009, 139, 471.   DOI   ScienceOn
13 Kim, H.; Kim, Y.; Joo, J. B.; Ko, J. W.; Yi, J. Micropor. Mesopor. Mat. 2009, 122, 283.   DOI   ScienceOn
14 Lee, Y.-J.; Park, D.-J.; Park, J.-Y.; Kim, Y. Sensors 2009, 8, 6154.   DOI
15 Howard, N.; Abell, C.; Blakemore, W.; Chessari, G.; Howard, S.; Murray, C. W.; Seavers, L. C.; van Montfort, R. L. J. Med. Chem. 2006, 49, 1346.   DOI   ScienceOn
16 Hiankik, T.; Wang, J. Electroanalysis 2009, 21, 1223.   DOI   ScienceOn
17 Baldrich, E.; Restrepo, A.; O'Sullivan, C. K. Anal. Chem. 2004, 76, 7053.   DOI   ScienceOn
18 Hamaguchi, N.; Ellington, A.; Stanton, M. Analy. Biochem. 2001, 294, 126.   DOI   ScienceOn
19 Smirnov, I.; Shafer, R. H. Biochem. 2000, 39, 1462.   DOI   ScienceOn
20 Holland, C. A.; Henry, A. T.; Whinna, H. C.; Church, F. C. FEBS Lett. 2000, 484, 87.   DOI   ScienceOn
21 Francois, L.; David, F. W. Physiol. Rev. 1954, 34, 722.   DOI
22 Nishino, A.; Suzuki, M.; Ohtani, H.; Motohashi, O.; Umezawa, K.; Nagura, H.; Yoshimoto, T. J. Neurotrauma 1993, 10, 167.   DOI   ScienceOn
23 Arai, T.; Miklossy, J.; Klegeris, A.; Guo, J. P.; McGeer, P. L. J. Neuropathol. Exp. Neurol. 2006, 65, 19.   DOI   ScienceOn
24 Wang, Y.; Liu, B. Langmuir 2009, 25, 12787.   DOI   ScienceOn
25 Zheng, J.; Chen, G.-F.; He, P.-G.; Fang, Y.-Z. Talanta 2010, 80, 1868.   DOI   ScienceOn