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http://dx.doi.org/10.5012/bkcs.2011.32.4.1221

A Simple and Sensitive High Performance Liquid Chromatography-Electrospray Ionization/Mass Spectrometry Method for the Quantification of Ethyl Pyruvate in Rat Plasma  

Kim, Hyun-Ji (Department of Molecular Science and Technology, Ajou University)
Kim, Seung-Woo (Department of Anatomy, Center for Advanced Medical Education, School of Medicine, Inha University)
Lee, Ja-Kyeong (Department of Anatomy, Center for Advanced Medical Education, School of Medicine, Inha University)
Yoon, Sung-Hwa (Department of Molecular Science and Technology, Ajou University)
Publication Information
Bulletin of the Korean Chemical Society / v.32, no.4, 2011 , pp. 1221-1227 More about this Journal
Abstract
Ethyl pyruvate (EP) is known as a scavenger of reactive oxygen species (ROS) in the body through its role in the donation of diketone groups to metals to form an EP-metal complex. In order to develop a method for the quantification of EP in biological media, a sensitive and specific, high-performance liquid chromatographyelectrospray ionization-mass spectrometry (HPLC-ESI/MS) method is used to determine the EP-alkali metal ion binding species. The analyte was separated on a ZORBOX SB-C8 ($3.5{\mu}m$, $30mm{\times}2.1mm$ I.D.) column and analyzed in selected ion monitoring (SIM) mode with a positive ESI interface using the m/z 255 $[2M + Na]^+$ ion. The method was validated over the concentration range of $0.5-60.0\;{\mu}g$/mL under 1/9 (v/v) of acetonitrile/methanol solvent system with flow rate 0.05 mL/min. The limit of quantification (LOQ) was $0.5{\mu}g$/mL.
Keywords
Ethyl pyruvate; Quantification; Liquid chromatography-mass spectrometry; Electrospray ionization; Sodium adduct formation;
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