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http://dx.doi.org/10.5012/bkcs.2010.31.8.2201

Synthesis and Chromatographic Characteristics of Multidentate Ligand-Boned Silica Stationary Phases  

Li, Rong (School of Chemical Engineering, Northwest University)
Wang, Yan (School of Chemical Engineering, Northwest University)
Chen, Guo-Liang (School of Chemical Engineering, Northwest University)
Shi, Mei (School of Chemical Engineering, Northwest University)
Wang, Xiao-Gang (School of Chemical Engineering, Northwest University)
Zheng, Jian-Bin (Institute of Analytical Science, Northwest University)
Publication Information
Bulletin of the Korean Chemical Society / v.31, no.8, 2010 , pp. 2201-2206 More about this Journal
Abstract
To improve the separation property and stability of metal chelate Cu(II) column, three new kinds of multidentate aminocarboxy silica columns with cation-exchange properties were synthesized using glutamic acid (Glu), glutamic acidbromoacetic acid (Glu-BAA), glutamic acid-bromosuccinic acid (Glu-BSUA) as ligands and silica gel as matrix. The standard proteins were separated with prepared chromatographic columns. The stationary phases exhibited the metal chelate property after fixing copper ion (II) on the synthesized multidentate ligand silica columns. The binding capacity of immobilized metal ion was related with the dentate number of multidentate ligands. Chromatographic behavior of proteins and the leakage of immobilized metal ion on multidentate chelate Cu(II) columns were affected by the dentate number of multidentate ligands and competitive elution system directly. The results showed that quinquedentate Glu-BSUA-Cu(II) column exhibited better chromatographic property and stability as compared with tridentate Glu-Cu(II) column, tetradentate Glu-BAA-Cu(II) column and commonly used IDA-Cu(II) column.
Keywords
Cation-exchange; Metal chelate; Multidentate ligand; Binding capacity; Leakage;
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