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http://dx.doi.org/10.5012/bkcs.2010.31.6.1568

Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles  

Lee, Yeon-Ji (Department of Chemistry, Korea University)
Park, Jong-Moon (Department of Chemistry, Korea University)
Huh, Ji-Young (Department of Chemistry, Korea University)
Kim, Min-Sik (Department of Chemistry, Korea University)
Lee, Je-Sun (Department of Chemistry, Korea University)
Palani, Arudra (Department of Chemistry, Korea University)
Lee, Kwang-Yeol (Department of Chemistry, Korea University)
Lee, Sang-Won (Department of Chemistry, Korea University)
Publication Information
Bulletin of the Korean Chemical Society / v.31, no.6, 2010 , pp. 1568-1572 More about this Journal
Abstract
The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathionemodified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/MS/MS experiments.
Keywords
Proteomics; Glutathione; Superparamagnetic; Nanoparticle; Separation;
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