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http://dx.doi.org/10.5012/bkcs.2010.31.6.1474

NTAㆍNi2+-Functionalized Quantum Dots for VAMP2 Labeling in Live Cells  

Yu, Mi-Kyung (Department of Life Science, GIST)
Lee, Su-Ho (Department of Life Science, GIST)
Chang, Sung-Hoe (Department of Biomedical Sciences, Seoul National University College of Medicine)
Jon, Sang-Yong (Department of Life Science, GIST)
Publication Information
Bulletin of the Korean Chemical Society / v.31, no.6, 2010 , pp. 1474-1478 More about this Journal
Abstract
An efficient method for labeling individual proteins in live cells is required for investigations into biological mechanisms and cellular processes. Here we describe the preparation of small quantum dots (QDs) that target membrane surface proteins bearing a hexahistidine-tag ($His_6$-tag) via specific binding to an nitrilotriacetic acid complex of nickel(II) ($NTA{\cdot}Ni^{2+}$) on the QD surfaces. We showed that the $NTA{\cdot}Ni^{2+}$-QDs bound to His-tag functionalized beads as a cellular mimic with high specificity and that QDs successfully targeted $His_6$-tagged vesicle-associated membrane proteins (VMAP) on cell surfaces. This strategy provides an efficient approach to monitoring synaptic protein dynamics in spatially restricted and confined biological environments.
Keywords
Quantum dot conjugates; Imaging probes; Labeling; Protein tracking;
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