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http://dx.doi.org/10.5012/bkcs.2010.31.5.1187

Construction of 1H-15N Double Resonance Solid-State NMR Probe for Membrane Proteins in Aligned Bicelles  

Park, Tae-Joon (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Kim, Ji-Sun (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Um, Seung-Hoon (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Kim, Yong-Ae (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
Publication Information
Bulletin of the Korean Chemical Society / v.31, no.5, 2010 , pp. 1187-1191 More about this Journal
Abstract
$^1H-^{15}N$ heteronuclear dipolar coupling solid-state NMR experiments on lipid bilayer or bicelle samples are very useful for the structural studies of membrane proteins. However, to study these biological samples using solid-state NMR, a specific probe with high efficiency and high capability is required. In this paper, we describe the optimized design, construction, and efficiency of a 400 MHz wide-bore $^1H-^{15}N$ solid-state NMR probe with 5-mm solenoidal rf coil for high power, multi-pulse sequence experiments, such as 2D PISEMA or 2D SAMMY.
Keywords
Solid-state NMR; Home-built probe; Membrane proteins; Bicelles; Bilayers;
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