Browse > Article
http://dx.doi.org/10.5012/bkcs.2010.31.01.157

Adsorptive Immobilization of Acetylcholine Esterase on Octadecyl Substituted Porous Silica: Optical Bio-analysis of Carbaryl  

Norouzy, Amir (Department of Biochemistry and Biophysics, Babol University of Medical Sciences)
Habibi-Rezaei, Mehran (School of Biology, College of Science, University of Tehran)
Qujeq, Durdi (Department of Biochemistry and Biophysics, Babol University of Medical Sciences)
Vatani, Maryam (School of Biology, College of Science, University of Tehran)
Badiei, Alireza (School of Chemistry, College of Science, University Tehran)
Publication Information
Bulletin of the Korean Chemical Society / v.31, no.1, 2010 , pp. 157-161 More about this Journal
Abstract
A sensory element against carbaryl, as a widely used pesticide was prepared based on adsorbed acetylcholine esterase (AChE) from Torpedo california. Octadecyl was substituted on macro-porous silica, confirmed by infra-red (IR) spectroscopy and quantitatively estimated through thermo-gravimetric analysis (TGA). Immobilization of the enzyme was achieved by adsorption on this support. Activity of the immobilization product was measured as a function of the loaded enzyme concentration, and maximum binding capacity of the support was estimated to be 43.18 nmol.mg-1. The immobilized preparations were stable for more than two months at storage conditions and showed consistency in continuous operations. Possible application of the immobilized AChE for quantitative analysis of carbaryl is proposed in this study.
Keywords
Bio-analysis; Acetylcholine esterase; Adsorption; Macro-porous silica; Carbaryl;
Citations & Related Records

Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Nunes, G. S.; Skladal, P.; Yamanaka, H.; Barcelo, D. Analytica Chimica Acta 1998, 362, 59.   DOI   ScienceOn
2 Mavrikou, S.; Flampouri, K.; Moschopoulou, G.; Mangana, O.; Michaelides, A.; Kintzios, S. Sensors 2008, 8(4), 2818.   DOI
3 Ashok, K.; Singh, D. S. Comp. Biochem. Physiol. 1998, 119, 97.   DOI   ScienceOn
4 Yazal, J. E.; Rao, S. N.; Mehl, A.; Slikker, W. J. Toxicol. Sci. 2001, 63, 223.   DOI   ScienceOn
5 Suwansa-ard, S.; Kanatharana, P.; Asawatreratanakul, P.; Limsakul, C.; Wongkittisuksa, B.; Thavarungkul, P. Biosens. Bioelectron. 2005, 21, 445.   DOI   ScienceOn
6 Barshan-Tashnizi, M.; Ahmadian, Sh.; Niknam, K.; Torabi, S. F.; Ranaei-Siadat, S. O. Biotechnol. Appl. Biochem. 2009, 52, 257.   DOI   ScienceOn
7 Nemat-Gorgani, M.; Karimian, K. Eur. J. Biochem. 1982, 123, 601.
8 Andrescu, S.; Avramescu, A.; Bala, C.; Magearu, V.; Marty, J. L. Anal. Bioanal.Chem. 2002, 374, 39.   DOI
9 Andreescu, S.; Barthelmebs, L.; Marty, J. L. Analytica Chimica Acta 2002, 464, 171.   DOI   ScienceOn
10 Flores, F.; Artigas, J.; Marty, J. L.; Valdes, F. Anal. Bioanal. Chem. 2003, 376(4), 476.   DOI
11 Andreescu, S.; Marty, J. L. Biomolecular Engineering 2006, 23(1), 1.   DOI   ScienceOn
12 Blevins, R. D.; Dunn, W. C. J. Agric. Food Chem. 1975, 23(3), 377.   DOI
13 Houghton, P. J.; Rena, Y.; Howes, M. J. Nat. Prod. Rep. 2006, 23, 181.   DOI   ScienceOn
14 Granmer, M. F. Neurotoxicology 1986, 7, 247.
15 Wills, J. H.; Jamson, E.; Coulston, F. Clinic. Toxicol. 1968, 1, 265.   DOI
16 Kumar, J.; Kumar Jha, S.; D'Souza, S. F. Biosensors and Bioelectronics 2006, 21, 2100.   DOI   ScienceOn
17 Sherma, J. J. Environ. Sci. Health B 2007, 42, 429.   DOI   ScienceOn
18 Bartolini, M.; Cavrini, V.; Andrisano, V. Analytical Biochemistry 2005, 342, 163.   DOI   ScienceOn
19 Skladal, P.; Fiala, M.; Krejci, J. Intern. J. Environ. Anal. Chem. 1996, 65, 139.   DOI   ScienceOn
20 Wink, T.; Van Zuilen, S. J.; Bult, A.; Bennekom, W. P. Analyst 1997, 122, 43R.   DOI   ScienceOn
21 Gooding, J. J.; Hibbert, D. B. Trends Anal. Chem. 1999, 18, 525.   DOI   ScienceOn
22 Pauliukaite, R.; Schoenleber, M.; Vadgama, P.; Brett, C. M. Biocatal. Biotransform. 2004, 22, 145.   DOI   ScienceOn
23 Sotiropoulou, S.; Chaniotakis, N. A. Biomaterials 2005, 26, 6771.   DOI   ScienceOn
24 Joshi, K. A.; Tang, J.; Haddon, R.; Wang, J.; Chen, W.; Mulchaldani, A. Electroanalysis 2005, 7, 54.
25 Hofstee, B. H. J. Biochem. Biophys. Res. Commun. 1973, 50, 751.   DOI   ScienceOn
26 Lin, Y. H.; Lu, F.; Wang, J. Electroanalysis 2004, 16, 145.   DOI   ScienceOn
27 Ellman, G. L.; Coutney, K. D.; Valentino, A.; Featherstone, R. M. Biochem. Pharmacol. 1961, 7, 88.   DOI   ScienceOn
28 Duncan, A. C.; Sefton, M. V.; Brash, J. L. Biomaterials 1997, 18, 1585.   DOI   ScienceOn
29 Hjerten, S. J. Chromatogr. 1973, 87, 325.   DOI   ScienceOn
30 Azari, F.; Hosseinkhani, S.; Nemat-Gorgani, M. Appl. Biochem. Biotechnol. 2001, 94, 265.   DOI   ScienceOn
31 Yongnian, N.; Dongxia, C.; Serge, K. Anal. Chim. Acta 2007, 588, 131.   DOI   ScienceOn
32 Andreescu, S.; Barthelmebs, L.; Marty, J. L. Anal. Chem. Acta 2002, 464, 171.   DOI   ScienceOn
33 Habibi-Rezaei, M.; Nemat-Gorgani, M. Appl. Biochem. Biotechnol. 2002, 97, 79.   DOI   ScienceOn
34 Valdes-Ramirez, G.; Cortina, M.; Ramirez-Silva, M. T.; Marty, J. L. J. Anal. Bioanal. Chem. 2008, 392, 699.   DOI
35 Abad, J. M.; Pariente, F.; Hernandez, L.; Abruna, H. D.; Lorenzo, E. Anal. Chem. 1998, 70, 2848.   DOI   ScienceOn
36 La Rosa, C.; Pariente, F.; Hernandez, L.; Lorenzo, E. Analytica Chimica Acta 1994, 295, 273.   DOI   ScienceOn
37 Santhoshkumar, P.; Karanth, S.; Shivanandappa, T. Fundam. Appl. Toxicol. 1996, 32, 23.   DOI   ScienceOn
38 Lee, H. S.; Kim, Y. A.; Chao, Y. A.; Lee, Y. T. Chemosphere 2002, 46, 571.   DOI   ScienceOn