Browse > Article
http://dx.doi.org/10.5012/bkcs.2010.31.10.2889

Performance Characteristic of the β-Human Chorionic Gonadotropin Assay on the Access 2 for Its Use in Doping Control  

Kang, Min-Jung (Integrated Omics Center, Korea Institute of Science and Technology)
Lee, Hye-Im (Doping control center, Korea Institute of Science and Technology)
Shin, Sang-Mi (Doping control center, Korea Institute of Science and Technology)
Yoo, Hye-Hyun (Department of Pharmacy, College of Pharmacy, Hanyang University)
Kwon, Oh-Seung (Doping control center, Korea Institute of Science and Technology)
Jin, Chang-Bae (Integrated Omics Center, Korea Institute of Science and Technology)
Chung, Bong-Chul (Integrated Omics Center, Korea Institute of Science and Technology)
Publication Information
Bulletin of the Korean Chemical Society / v.31, no.10, 2010 , pp. 2889-2892 More about this Journal
Abstract
The objective of this study was to validate performance characteristics of the Access 2 (Beckman coulter) system for hCG assays for use as a confirmation test for doping control. The Access 2 assay was linear up to 500 IU/L. The correlation coefficient was higher than 0.999, and the sensitivity of the linearity was 0.82. There were no false positive or false negative cases. LOD was 0.59 IU/L. The method was robust when performed by different people. Repeatability and reproducibility were below 7%. We compared reproducibility and recoveries of Access 2 and Elecsys 2010. Access 2 demonstrated higher reproducibility than Elecsys 2010. Recoveries (accuracy) of Access 2 were between 85 and 105%. Recoveries for Elecsys 2010 were between 91 and 104%.
Keywords
$\beta$-hCG; Access 2; Doping control; Method evaluation; Confirmation method;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Cole, L. A. Clin. Chem. 1997, 43, 2233.
2 Stenman, U. H.; Tiitinen, A.; Alfthan, H.; Valmu, L. Hum. Reprod. 2006, 12, 769.   DOI   ScienceOn
3 Cowan, D. A.; Kicman, A. T.; Walker, C. J.; Wheeler, M. J. J. Endocrinol. 1991, 131, 147.   DOI   ScienceOn
4 De Boer, D.; De Jong, E. G.; Van Rossum, J. M.; Maes, R. A. A. Int. J. Sports Med. 1991, 12, 46.   DOI
5 Kicman, A. T.; Brooks, R. V.; Cowan, D. A. Br. J. Sports Med. 1991, 25, 73.   DOI   ScienceOn
6 Laidler, P.; Cowan, D. A.; Hider, R. C.; Kicman, A. T. Clin. Chem. 1994, 40, 1306.
7 Gam, L. H.; Tham, S. Y.; Latiff, A. J. Chromatogr. B Analyst. Technol. Biomed. Life Sci. 2003, 792, 187.   DOI   ScienceOn
8 Delbeke, F. T.; Van Eenoo, P.; De Backer, P. Int. J. Sports Med. 1998, 19, 287.   DOI   ScienceOn
9 World Anti-doping Agency; http://www.wada-ama.org.
10 Stenman, U.-H.; Unkila-Kallio, L.; Korhonen, J.; Alfthan, H. Clin. Chem. 1997, 43, 1293.
11 Shahzad, K.; Kim, D.-H.; Kang, M.-J. Clin. Biochem. 2007, 40, 1259.   DOI   ScienceOn
12 Guidelines for the validation and verification of chemical test methods, NATA Guidelines Technical note 17.