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http://dx.doi.org/10.5012/bkcs.2009.30.9.1973

Interaction of ct-DNA with 2,4-Dihydroxy Salophen  

Azani, Mohammad-Reza (Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan)
Hassanpour, Azin (Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan)
Bordbar, Abdol-Khalegh (Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan)
Mirkhani, Valiollah (Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan)
Publication Information
Bulletin of the Korean Chemical Society / v.30, no.9, 2009 , pp. 1973-1977 More about this Journal
Abstract
In the present study, at first, 2,4-Dihydroxy Salophen (2,4-DHS), has been synthesized by combination of 1, 2-diaminobenzene and 2,4-dihydroxybenzaldehyde in a solvent system. This ligand containing meta-quinone functional groups were characterized using UV-Vis and IR spectroscopies. Subsequently, the interaction between native calf thymus deoxyribonucleic acid (ct-DNA) and 2,4-DHS, was investigated in 10 mM Tris/HCl buffer solution, pH 7.2, using UV-visible absorption and fluorescence spectroscopies, thermal denaturation technique and viscosity measurements. From spectrophotometric titration experiments, the binding constant of 2,4-DHS with ct-DNA was found to be (1.1 ${\pm}\;0.2)\;{\times}\;10^4\;M^{-1}.$ The fluorescence study represents the quenching effect of 2,4-DHS on bound ethidium bromide to DNA. The quenching process obeys linear Stern-Volmer equation in extended range of 2,4-DHS concentration. Thermal denaturation experiments represent the increasing of melting temperature of DNA (about 3.5 ${^{\circ}C}$) due to binding of 2,4-DHS. These results are consistent with a binding mode dominated by interactions with the groove of ct-DNA.
Keywords
DNA; Schiff base; Interaction; Fluorescence; UV-Vis;
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