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http://dx.doi.org/10.5012/bkcs.2009.30.8.1827

Isolation of RNA Aptamers Targeting HER-2-overexpressing Breast Cancer Cells Using Cell-SELEX  

Kang, Hye-Suk (Global Research Laboratory for RNAi Medicine, Department of Chemistry and BK21 School of Chemical Materials Science, Sungkyunkwan University)
Huh, Yong-Min (Department of Radiology & Department of Biochemistry and Molecular Biology, Yonsei University)
Kim, So-Youn (Department of Biomedical Engineering, Dongguk University)
Lee, Dong-ki (Global Research Laboratory for RNAi Medicine, Department of Chemistry and BK21 School of Chemical Materials Science, Sungkyunkwan University)
Publication Information
Bulletin of the Korean Chemical Society / v.30, no.8, 2009 , pp. 1827-1831 More about this Journal
Abstract
Ligand molecules that can recognize and interact with cancer cell surface marker proteins with high affinity and specificity should greatly aid the development of novel cancer diagnostics and therapeutics. HER-2/ErbB2/Neu (HER-2), a member of the epidermal growth factor receptor family, is specifically overexpressed on the surface of breast cancer cells and serves as both a useful biomarker and a therapeutic target for breast cancer. In this study, we aimed to isolate RNA aptamers that specifically bind to a HER-2-overexpressing human breast cancer cell line, SK-BR-3, using Cell-SELEX strategy. The selected aptamers showed strong affinity to SK-BR-3, but not to MDAMB- 231, a HER-2-underexpressing breast cancer cell line. In addition, we confirmed the specific targeting of HER-2 receptor by aptamers using an unrelated mouse cell line overexpressing human HER-2 receptor. The HER-2-targeting RNA aptamers could become a useful reagent for the development of breast cancer diagnostics and therapeutics.
Keywords
HER-2; Cell-SELEX; Aptamer; Breast cancer; siRNA;
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