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http://dx.doi.org/10.5012/bkcs.2008.29.10.2017

Real-time Acquisition of Three Dimensional NMR Spectra by Non-uniform Sampling and Maximum Entropy Processing  

Jee, Jun-Goo (Center for Priority Areas, Tokyo Metropolitan University)
Publication Information
Bulletin of the Korean Chemical Society / v.29, no.10, 2008 , pp. 2017-2022 More about this Journal
Abstract
Of the experiments to shorten NMR measuring time by sparse sampling, non-uniform sampling (NUS) is advantageous. NUS miminizes systematic errors which arise due to the lack of samplings by randomization. In this study, I report the real-time acquisition of 3D NMR data using NUS and maximum-entropy (MaxEnt) data processing. The real-time acquisition combined with NUS can reduce NMR measuring time much more. Compared with multidimensional decomposition (MDD) method, which was originally suggested by Jaravine and Orekhov (JACS 2006, 13421-13426), MaxEnt is faster at least several times and more suitable for the realtime acquisition. The designed sampling schedule of current study makes all the spectra during acquisition have the comparable resulting resolutions by MaxEnt. Therefore, one can judge the quality of spectra easily by examining the intensities of peaks. I report two cases of 3D experiments as examples with the simulated subdataset from experimental data. In both cases, the spectra having good qualitie for data analysis could be obtained only with 3% of original data. Its corresponding NMR measuring time was 8 minutes for 3D HNCO of ubiquitin.
Keywords
Non-uniform sampling; Maximum entropy processing; NMR; Real-time acquisition
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