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http://dx.doi.org/10.5012/bkcs.2007.28.5.745

Development of Isotope Dilution-Liquid Chromatography/Tandem Mass Spectrometry as a Candidate Reference Method for the Determination of Folic Acid in Infant Milk Formula  

Jung, Min-Young (Department of Chemistry, Yonsei University)
Kim, Byung-Joo (Division of Metrology for Quality Life, Korea Research Institute of Standards and Science)
Boo, Doo-Wan (Department of Chemistry, Yonsei University)
So, Hun-Young (Division of Metrology for Quality Life, Korea Research Institute of Standards and Science)
Publication Information
Bulletin of the Korean Chemical Society / v.28, no.5, 2007 , pp. 745-750 More about this Journal
Abstract
An isotope dilution-liquid chromatography/tandem mass spectrometric method was developed as a candidate reference method for the accurate determination of folic acid in infant milk formula. Sample was spiked with 13C5-folic acid and then extracted with phosphate buffer (pH 6) solution. The extract was further cleaned up by deproteinization followed by a C18 solid-phase extraction cartridge. The extract was analyzed by using LC/ ESI/MS/MS with selectively monitoring the collisionally induced dissociation channels of m/z 442 → m/z 295 and m/z 447 → m/z 295, which are the neutral glutamyl loss from the [M+H]+ ions of folic acid and 13C5-folic acid, respectively. LC/MS/MS chromatograms showed substantially reduced background from chemical noises compared to LC/MS chromatograms. Repeatability and reproducibility studies showed that the LC/MS/ MS method is a reliable and reproducible method which can provide less than 1.5 relative percentage of method precision.
Keywords
Folic acid; Infant formula; ID-LC/MS; Method validation; Reference method;
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