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http://dx.doi.org/10.5012/bkcs.2007.28.3.427

Proteome Analysis of Vernalization-Treated Arabidopsis thaliana by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry  

Cho, Mi-Ran (Department of Chemistry, Seoul National University)
Lee, Kyung-Hyeon (Department of Chemistry, Seoul National University)
Hyun, You-Bong (Department of Biological Sciences, Seoul National University)
Lee, Il-Ha (Department of Biological Sciences, Seoul National University)
Kim, Hie-Joon (Department of Chemistry, Seoul National University)
Publication Information
Bulletin of the Korean Chemical Society / v.28, no.3, 2007 , pp. 427-431 More about this Journal
Abstract
In order to gain insight into the molecular changes at the protein level in plants exposed to low temperature for a long period of time (vernalization), proteome analyses of vernalization-treated Arabidopsis thaliana have been carried out by two-dimensional gel electrophoresis followed by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. Fourteen proteins including ATP binding/GTP binding/translation elongation factor and glycine-rich RNA-binding protein 7 (GRP7) showed differential expression in vernalization-treated Arabidopsis thaliana. GRP7 showed the most dramatic increase in expression suggesting its involvement in response to vernalization treatment.
Keywords
Arabidopsis thaliana; Vernalization; MALDI-TOF MS; GRP7;
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