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http://dx.doi.org/10.5012/bkcs.2007.28.12.2329

DNA Cleavage Induced by the Reaction of Salsolinol with Cu,Zn-Superoxide Dismutase  

Kang, Jung-Hoon (Department of Genetic Engineering, Cheongju University)
Publication Information
Bulletin of the Korean Chemical Society / v.28, no.12, 2007 , pp. 2329-2332 More about this Journal
Abstract
Salsolinol, endogenous neurotoxin, is known to be involved in the pathogenesis of Parkinson's disease (PD). In the present study, we have investigated the oxidative damage of DNA induced by the reaction of salsolinol with Cu,Zn-SOD. When plasmid DNA incubated with salsolinol and Cu,Zn-SOD, DNA cleavage was proportional to the concentrations of salsolinol and Cu,Zn-SOD. The salsolinol/Cu,Zn-SOD system-mediated DNA cleavage was significantly inhibited by radical scavengers such as mannitol, ethanol and thiourea. These results indicated that free radicals might participate in DNA cleavage by the salsolinol/Cu,Zn-SOD system. Spectrophotometric study using a thiobarbituric acid showed that hydroxyl radical formation was proportional to the concentration of salsolinol and was inhibited by radical scavengers. These results indicated that hydroxyl radical generated in the reaction of salsolinol with Cu,Zn-SOD was implicated in the DNA cleavage. Catalase and copper chelators inhibited DNA cleavage and the production of hydroxyl radicals. These results suggest that DNA cleavage is mediated in the reaction of salsolinol with Cu,Zn-SOD via the generation of hydroxyl radical by a combination of the oxidation reaction of salsolinol and Fenton-like reaction of free copper ions released from oxidatively damaged SOD.
Keywords
Salsolinol; Copper,zinc-superoxide dismutase; DNA cleavage;
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1 Ikeda, H.; Markey, C. J.; Markey, S. P. Brain Res. 1993, 575, 285   DOI   ScienceOn
2 Moser, A.; Kompf, D. Life Sci. 1992, 50, 1885   DOI   ScienceOn
3 Niwa, T.; Takeda, T.; Yoshizumi, H.; Tatematsu, A.; Yoshida, M.; Dosterdt, P.; Naoi, M.; Nagatsu, T. Biochem. Biophys. Res. Commun. 1991, 177, 603   DOI   ScienceOn
4 Ohta, S.; Kohno, M.; Makino, Y.; Tachikawa, O.; Hirobe, M. Biomed. Res. 1987, 8, 453   DOI
5 Kim, H. J.; Yoon, H. R.; Washington, S.; Chang, I. I.; Oh, Y. J.; Surh, Y. J. Neurosci. Lett. 1997, 238, 95   DOI   ScienceOn
6 Kim, H. J.; Soh, Y.; Jang, J. H.; Lee, J. S.; Oh, Y. J.; Surh, Y. J. Mol. Pharmacol. 2001, 60, 440
7 Ebadi, M. Methods Enzymol. 1991, 205, 363   DOI
8 Ebadi, M.; Kumari, M. V. R.; Hiramatsu, M.; Hao, R.; Pfeiffer, R. F.; Rojas, P. Restor. Neurol. Neurosci. 1998, 12, 103
9 Kumari, M. V. R.; Hiramatsu, M.; Ebadi. M. Cell Mol. Biol. 2000, 46, 627
10 Bowling, A. C.; Schulz, J. B.; Brown, R. H. Jr.; Beal, M. F. Neurochem. 1993, 61, 2322   DOI   ScienceOn
11 Sagripanti, J. L.; Swicord, M. L.; Davis, C. C. Radiat. Res. 1987, 110, 219   DOI   ScienceOn
12 Goldstein, S.; Czapski, G. J. Am. Chem. Soc. 1986, 108, 2244   DOI   ScienceOn
13 Gutteridge, J. M. C.; Halliwell, B. Biochem. Pharmacol. 1982, 31, 2801   DOI   ScienceOn
14 Imlay, J. A.; Chin, S. M.; Linn, S. Science 1988, 240, 640   DOI
15 Prutz, W. A. Radiat. Environ. Biophys. 1984, 23, 7   DOI   ScienceOn
16 Sagripanti, J.-L.; Kraemer, K. H. J. Biol. Chem. 1989, 264, 1729
17 Jenner, P.; Dexter, D. T.; Sian, J.; Schapira, A. H.; Marsden, C. D. Ann. Neural. 1992, 32, Suppl: 582
18 Ihara, Y.; Nobukuni, K.; Takata, H.; Hayabara, T. Neurol. Res. 2005, 27, 105   DOI   ScienceOn
19 Kang, J. H.; Kim, S. M. Mol. Cells 1997, 7, 553
20 Kim, K. S.; Choi, S. Y.; Kwon, H. Y.; Won, M. H.; Kang, T. C.; Kang, J. H. Free Radical. Biol. Med. 2002, 32, 544   DOI   ScienceOn
21 Choi, S. Y.; Kwon, H. Y.; Kwon, O. B.; Kang, J. H. Biochim. Biophys. Acta 1999, 1472, 651   DOI   ScienceOn
22 Martilla, R. J.; Lorezo, H.; Rinne, U. K. J. Neurrol Sci. 1988, 86, 32
23 Saggu, H.; Cooksey, J.; Dexter, D.; Wells, F. R.; Lees, A.; Jenner, P.; Marsden, C. D. J. Neurochem. 1989, 53, 692   DOI
24 Feldberg, R. S.; Carao, J. A.; Paradise, R. J. Free Radic. Biol. Med. 1985, 1, 459   DOI   ScienceOn
25 Chiou, S. H. J. Biochem. (Tokyo) 1983, 94, 1259   DOI
26 Rowley, D. A.; Halliwell, B. Arch. Biochem. Biophys. 1983, 225, 279   DOI   ScienceOn
27 Reed, C. J.; Douglas, K. T. Biochem. Biophys. Res. Commun. 1989, 162, 1111   DOI   ScienceOn
28 Hensley, K.; Carney, J. M.; Mattson, M. P.; Aksenova, M.; Harris, M.; Wu, J. F.; Floyd, R. A.; Butterfield, D. A. Proc. Natl. Acad. Sci. USA 1994, 91, 3270   DOI   ScienceOn
29 Kang, J. H. J. Biochem. Mol. Biol. 2007, 40, 684   DOI
30 Pall, H. S.; Williams, A. C.; Blake, D. R.; Lunec, J.; Gutteridge, J. M.; Hall, M.; Taylor, A. Lancet. 1987, 2, 238
31 Multhaup, G.; Schlicksupp, A.; Hesse, L.; Behler, D.; Ruppert, T.; Masters, C. L.; Beyreuther, K. Science 1996, 271, 1406   DOI   ScienceOn
32 Cerruti, P. A. Science 1984, 227, 375
33 Kang, J. H. Bull. Korean Chem. Soc. 2005, 26, 1251   DOI   ScienceOn
34 Kim, S. M.; Kang, J. H. Mol. Cells 1997, 7, 120
35 Tainer, J. A.; Gertzoff, E. D.; Richardson, J. S.; Richardson, D. C. Nature 1983, 306, 284   DOI   ScienceOn
36 Stoewe, R. S.; Prutz, W. A. Free Radical Biol. Med. 1987, 3, 97   DOI   ScienceOn
37 Bindoli, A.; Rigobello, M. P.; Deeble, D. J. Free Radic. Bio. Med. 1992, 13, 391   DOI   ScienceOn