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http://dx.doi.org/10.5012/bkcs.2006.27.3.413

Size Characterization of Sodium Hyaluronate by Field Programming Frit Inlet Asymmetrical Flow Field-Flow Fractionation/Multiangle Light Scattering  

Kim, Hoon-joo (LG Life Sciences, Ltd./R&D Park)
Lee, Hee-jeong (Department of Chemistry, Yonsei University)
Moon, Myeong-Hee (Department of Chemistry, Yonsei University)
Publication Information
Bulletin of the Korean Chemical Society / v.27, no.3, 2006 , pp. 413-418 More about this Journal
Abstract
Sodium hyaluronate (NaHA), water soluble polymer having ultra-high molecular weight, is characterized by using on-line frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) and multiangle light scattering (MALS). This study demonstrates the capability of power programming FI-AFlFFF for the separation of NaHA and the applicability of FI-AFlFFF with MALS for the characterization of molecular weight distribution and their structural information. Since sample injection and relaxation in FI-AFlFFF are achieved by using hydrodynamic relaxation, separation of high molecular weight polymers can be achieved smoothly without halting the separation flow. Experiments are carried out with the two different NaHA products (a raw NaHA sample and a thermally degraded NaHA product) and molecular weight distribution and conformations in solution are determined. Influence of sample filtration on the change of molecular weight distribution is also discussed.
Keywords
Field-flow fractionation; Frit inlet asymmetrical flow field-flow fractionation; Molecular weight determination; Multiangle light scattering; Field programming;
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