Browse > Article
http://dx.doi.org/10.5012/bkcs.2006.27.1.071

Preparation and Characterization of Monolithic Poly(methacrylic acid - ethylene glycol dimethacrylate) Columns for High Performance Liquid Chromatography  

Yan, Hong-yuan (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University)
Row, Kyung-Ho (Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University)
Publication Information
Bulletin of the Korean Chemical Society / v.27, no.1, 2006 , pp. 71-76 More about this Journal
Abstract
Porous polymer monolithic columns were prepared by the direct free radical copolymerization of methacrylic acid and ethylene glycol dimethacrylate within the confines of a chromatographic column in the presence of toluene-dodecanol as a porogenic solvent. The separation characteristics of the monolithic columns were tested by a homologous series of xanthine derivatives, theophylline and caffeine. The effects of the polymerization mixture composition and polymerization condition, mobile phase composition, flow rate and temperature on the retention times and separation efficiencies were investigated. The results showed that the selection of correct porogenic solvents and appropriate polymerization conditions are crucial for the preparation of the monolithic stationary phases. The separation efficiency was only extremely weakly dependent on flow rate and temperatures. Hydrogen-bonding interaction played an important role in the retention and separation. Compared with conventional particle columns, the monolithic column exhibited good stability, ease of regeneration, high separation efficiency and fast analysis.
Keywords
Monolithic column; In-situ polymerization; Theophylline; Caffeine;
Citations & Related Records

Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Minakuchi, H.; Nakanishi, K.; Soga, N.; Ishizuka, N.; Tanaka, N. Anal. Chem. 1996, 68, 3498   DOI   ScienceOn
2 Tanaka, N.; Kobayashi, H.; Nakanishi, K.; Minakuchi, H.; Ishizuka, N. Anal. Chem. 2001, 73, 420A
3 Nakanishi, K. J. Porous Materials 1997, 4, 67   DOI   ScienceOn
4 Ishizuka, N.; Kobayashi, H.; Minakuchi, H.; Nakanishi, K.; Hirao, K.; Hosoya, K.; Ikegami, T.; Tanaka, N. J. Chromatogr. A 2002, 960, 85   DOI   ScienceOn
5 Ikegami, T.; Tanaka, N. Anal. Tech. 2004, 8, 527
6 Gusev, I.; Huang, X.; Horvath, C. J. Chromatogr. A 1999, 855, 273   DOI   ScienceOn
7 Sykora, D.; Svec, F.; Frechet, J. M. J. J. Chromatogr. A 1999, 852, 297   DOI   ScienceOn
8 Fan, Y.; Feng, Y. Q.; Da, S. L.; Shi, Z. G. Analytica Chimica Acta 2004, 523, 251   DOI   ScienceOn
9 Svec, F. J. Sep. Sci. 2004, 27, 747   DOI   ScienceOn
10 Ko, J. H.; Kang, G. W.; Seo, Y. J.; Cheong, W. J. Bull. Korean Chem. Soc. 2004, 25, 1589   DOI   ScienceOn
11 Asperger, A.; Efer, J.; Koal, T.; Engewald, W. J. Chromatogr. A 2002, 960, 109   DOI   ScienceOn
12 Yin, J. F.; Yang, G. L.; Chen, Y. J. Chromatogr. A 2005, 1090, 68   DOI   ScienceOn
13 Wang, D. X.; Hong, S. P.; Row, K. H. Bull. Korean Chem. Soc. 2004, 25, 357   DOI   ScienceOn
14 Kimura, H.; Tanigawa, T.; Morisaka, H.; Ikegami, T.; Hosoya, K.; Ishizuka, N.; Minakuchi, H.; Nakanishi, K.; Ueda, M.; Cabrera, K.; Tanaka, N. J. Sep. Sci. 2004, 27, 897   DOI   ScienceOn
15 Liu, H. Y.; Row, K. H.; Yang, G. L. Chromatographia 2005, 61, 429   DOI
16 Chankvetadze, B.; Yamamoto, C.; Okamoto, Y. Chem. Lett. 2003, 32, 850   DOI   ScienceOn
17 Xu, Q.; Mori, M.; Tanaka, K.; Ikedo, M.; Hu, W. J. Chromatogr. A 2004, 1026, 191   DOI   ScienceOn
18 Tanaka, N.; Kimura, H.; Tokuda, D.; Hosoya, K.; Ikegami, T.; Ishizuka, N.; Minakuchi, H.; Nakanishi, K.; Shintani, Y.; Furuno, M.; Cabrera, K. Anal. Chem. 2004, 76, 1273   DOI   ScienceOn
19 Jin, W.; Fu, H.; Huang, X.; Xiao, H.; Zou, H. Electrophoresis 2003, 24, 3172   DOI   ScienceOn
20 Allen, D.; Rassi, Z. J. Chromatogr. A 2004, 1029, 239   DOI   ScienceOn
21 Minakuchi, H.; Nakanishi, K.; Soga, N.; Ishizuka, N.; Tanaka, N. J. Chromatogr. A 1998, 797, 121   DOI   ScienceOn
22 Motokawa, M.; Kobayashi, H.; Ishizuka, N.; Minakuchi, H.; Nakanishi, K.; Jinnai, H.; Ikegami, T.; Tanaka, N. J. Chromatogr. A 2002, 961, 53   DOI   ScienceOn
23 Lubda, D.; Lindner, W. J. Chromatogr. A 2004, 1036, 135   DOI   ScienceOn
24 Svec, F.; Peters, E. C.; Sykora, D.; Yu, C.; Frechet, J. M. J. J. High Resolut. Chromatogr. 2000, 23, 3   DOI   ScienceOn
25 Cabrera, K. J. Sep. Sci. 2004, 27, 843   DOI   ScienceOn