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http://dx.doi.org/10.5012/bkcs.2006.27.10.1655

[Ru(phen)2(dppz)]2+ Assemble on the Surface of the SDS Micelle and Its Application for the Determination of DNA  

Chen, Fang (Department of Chemistry, Huazhong University of Science & Technology)
Huang, Jianping (College of Chemistry & Environmental Engineering, Yangtze University)
He, Zhike (College of Chemistry & Molecular Sciences, Wuhan University)
Publication Information
Bulletin of the Korean Chemical Society / v.27, no.10, 2006 , pp. 1655-1658 More about this Journal
Abstract
The solution of $[Ru(phen)_2(dppz)]^{2+}$ and SDS has high Resonance Light Scattering (RLS) signals due to $[Ru(phen)_2(dppz)]^{2+}$ assemble on the surface of the SDS micelle. Because of the high affinity ($KB\geq10^6\;L\;mol^{-1}$) between $[Ru(phen)_2(dppz)]^{2+}$ and DNA, the adding of DNA in the solution of $[Ru(phen)_2(dppz)]^{2+}$-SDS makes the dissociation of $[Ru(phen)_2(dppz)]^{2+}$-SDS, and results in decreasing of the RLS signals and increasing of the absorbance. Based on this, a novel method is proposed for DNA assay. Under optimum condition, good linear relationship was obtained within the concentration range of 0.018-1.26 $\mu g\;mL^{-1}$, the linear equation is $I_{RLS}$ = 504.8-348.8 c (c: $\mu g\;mL^{-1}$) and the correlation coefficient (r) is 0.9992. The detect limit for calf thymus DNA is 8.6 ng $mL^{-1}$. The proposed method was successful applied to determine the extracted colibacillus plasmid DNA.
Keywords
$[Ru(phen)_2(dppz)]^{2+}$; SDS; DNA; Resonance light scattering;
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