Browse > Article
http://dx.doi.org/10.5012/bkcs.2005.26.8.1255

Aggregation of α-Synuclein Induced by Oxidized Catecholamines as a Potential Mechanism of Lewy Body  

Kim, Kyung-Sik (Department of Genetic Engineering, Cheongju University)
Kang, Jung-Hoon (Department of Genetic Engineering, Cheongju University)
Publication Information
Bulletin of the Korean Chemical Society / v.26, no.8, 2005 , pp. 1255-1259 More about this Journal
Abstract
Lewy bodies (LBs) are neuronal inclusions that are closely related to Parkinson's disease (PD). The filamentous component of LB from patients with PD contains biochemically altered $\alpha$-synuclein. We have investigated the effect of the oxidized products of catecholamines on the modification of $\alpha$-synuclein. When $\alpha$-synuclein was incubated with the oxidized 3,4-dihydroxyphenylalanine (L-DOPA) or dopamine, the protein was induced to be aggregated. The oxidized catecholamine-mediated $\alpha$-synuclein aggregation was enhanced by copper ion. Radical scavengers, azide and N-acetyl cysteine significantly prevented the oxidized catecholamine-mediated $\alpha$-synuclein aggregation. The results suggest that free radical may play a role in $\alpha$-synuclein aggregation. Exposure of $\alpha$-synuclein to the oxidized products of catecholamines led to the formation of dityrosine. Antioxidant dipeptides carnosine, homocarnosine and anserine significantly protected $\alpha$-synuclein from the aggregation induced by the oxidized products of catecholamines.
Keywords
Lewy body; $\alpha$-Synuclein; L-DOPA; Carnosine;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Takeda, A.; Hashimoto, M.; Mallory, M.; Sundsumo, M.; Hansen, L.; Sisk, A.; Masliah, E. Lab. Invest. 1998, 78, 1169-1177
2 Pileblad, E.; Slivka, A.; Bratvold, D.; Cohen, G. Arch. Biochem. Biophys. 1988, 263, 447-452   DOI   ScienceOn
3 Takeda, A.; Mallory, M.; Sundsumo, M.; Honer, W.; Hansen, L.; Masliah, E. Am. J. Pathol. 1998, 152, 367-372
4 Spillantini, M. G.; Schmit, M. L.; Lee, V.-M.; Trojanowski, J. Q.; Jakes, R.; Goedert, M. Nature 1997, 388, 839-840   DOI   ScienceOn
5 Wakabayashi, K.; Matsumoto, K.; Takayama, K.; Yoshimoto, M.; Takahashi, H. Neurosci. Lett. 1997, 239, 45-48   DOI   ScienceOn
6 Irizarry, M. C.; Growdon, W.; Gomez-Isla, T.; Newell, K.; George, J. M.; Clayton, D. F.; Hyman, B. T. J. Neuropathol. Exp. Neurol. 1998, 57, 334-337   DOI
7 Baba, M.; Nakajo, S.; Tu, P.-H.; Tomita, T.; Nakaya, K.; Lee, V. M.; Trojanowski, J. Q.; Iwatsubo, T. Am. J. Pathol. 1998, 152, 879-884
8 Paik, S. R.; Shin, H. J.; Lee, J. H. Arch. Biochem. Biophys. 2000, 378, 269-277   DOI   ScienceOn
9 Choi, S. Y.; Kwon, H. Y.; Kwon, O. B.; Kang, J. H. Biochim. Biophys. Acta 1999, 1472, 651-657   DOI   ScienceOn
10 Goedert, M.; Spillantini, M. G.; Davies, S. W. Curr. Opin. Neurobiol. 1998, 8, 619-632   DOI   ScienceOn
11 Jenner, P. Pathol. Biol. (Paris) 1996, 44, 57-64
12 Dexter, D. T.; Jenner, P.; Schapira, A. H.; Marsden, C. D. Ann. Neurol. 1992, 32 Suppl, S94-100   DOI   ScienceOn
13 Jellinger, K. A.; Kienzl, E.; Rumpelmair, G.; Paulus, W.; Riederer, P.; Stachelberger, H.; Youdim, M. B.; Ben-Shachar, D. Adv. Neurol. 1993, 60, 267-272
14 Waite, J. H. Comp. Biochem. Physiol. B 1990, 97, 19-29   DOI   ScienceOn
15 Miller, D. M.; Buettner, G. R.; Aust, S. D. Free Radic. Biol. Med. 1990, 8, 95-108   DOI   ScienceOn
16 Graham, D. G. Mol. Pharmacol. 1978, 14, 633-643
17 Graham, D. G.; Tiffany, S. M.; Bell, W. R. Jr.; Gutknecht, W. F. Mol. Pharmacol. 1978, 14, 644-653
18 Ilic, T.; Jovanovic, M.; Jovicic, A.; Tomovic, M. Funct. Neurol. 1999, 14, 141-147
19 Stokes, A. H.; Hastings, T. G.; Vrana, K. E. J. Neurosci. Res. 1999, 55, 659-665   DOI   ScienceOn
20 Jones, D. C.; Gunasekar, P. G.; Borowitz, J. L.; Isom, G. E. J. Neurochem. 2000, 74, 2296-2304   DOI
21 Sutton, H. C.; Winterbourn, C. C. Free Radic. Biol. Med. 1989, 6, 53-60   DOI   ScienceOn
22 Kang, J. H. Bull. Korean Chem. Soc. 2004, 25, 625-628   DOI   ScienceOn
23 Stadtman, E. R. Ann. Rev. Bichem. 1993, 62, 792-821
24 Kohen, R.; Yamamoto, Y.; Cundy, K. C.; Ames, B. N. Proc. Natl. Acad. Sci. USA 1988, 85, 3175-3179   DOI   ScienceOn
25 O'Dowd, J. J.; Robins, D. J.; Miller, D. J. Biochem. Biophys. Acta 1988, 967, 241-249   DOI   ScienceOn
26 Kang, J. H. Bull. Korean Chem. Soc. 2005, 26, 178-180   DOI   ScienceOn
27 Harris, R. C.; Marlin, D. J.; Dunnett, M.; Snow, D.; Hultman, E. Comp. Biochem. Physiol. A 1990, 97, 249-251   DOI   ScienceOn
28 Chan, W. K. M.; Decker, E. A.; Lee, J. B.; Butterfield, D. A. J. Agric. Food Chem. 1994, 42, 1407-1410   DOI   ScienceOn
29 Halliwell, B. Acta Neurol. Scand. 1989, 126, 23-33
30 Youdim, M. B.; Ben-Shachar, D.; Riederer, P. Acta Neurol. Scand. 1989, 126, 47-54
31 Jenner, P.; Olanow, C. W. Neurology 1996, 47, S161-170   DOI   ScienceOn
32 Sagripant, J. L.; Kraemer, K. H. J. Biol. Chem. 1989, 264, 1729- 1734
33 Paik, S. R.; Shin, H. J.; Lee, J. H.; Chang, C. S.; Kim, J. Biochem. J. 1999, 340, 821-828   DOI
34 Markesbery, W. R.; Carney, J. M. Brain Pathol. 1999, 9, 133-146   ScienceOn
35 Kang, J. H.; Kim, S. M. Mol. Cells 1997, 7, 553-558
36 O'Connell, M. J.; Peters, T. J. Chem. Phys. Lipids 1987, 45, 241- 249   DOI   ScienceOn
37 Pall, H. S.; Williams, A. C.; Blake, D. R.; Lunec, J.; Gutteridge, J. M.; Hall M.; Taylor A. Lancet. 1987, 2, 238-241
38 Hashimoto, M.; Hsu, L. J.; Xia, Y.; Takeda, A. M.; Sundsumo, M.; Masliah, E. Neuroreport 1999, 10, 717-721   DOI
39 Dexter, D. T.; Cater, C. J.; Wells, F. R.; Javoy-Agid, F.; Agid, Y.; Lees, A.; Jenner, P.; Marsden, C. D. J. Neurochem. 1989, 52, 381- 389   DOI
40 Melamed, E.; Offen, D.; Shirvan, A.; Djaldetti, R; Barzilai, A.; Ziv, I. Ann. Neurol. 1998, 44, S149-154   DOI   ScienceOn
41 Multhaup, G.; Schlicksupp, A.; Hesse, L.; Beher, D.; Ruppert, T.; Masters, C. L.; Beyreuther, K. Science 1996, 271, 1406- 1409   DOI   ScienceOn
42 Spillantini, M. G.; Crowther, R. A.; Jakes, R.; Hasegawa, M.; Goedert, M. Proc. Natl. Acad. Saci. U.S.A. 1998, 95, 6469- 6473   DOI
43 Smythies, J. R. Schizophr. Res. 1997, 24, 357-364   DOI   ScienceOn
44 Sian, J.; Dexter, D. T.; Lees, A. J.; Daniel, S.; Agid, Y.; Javoy- Agid, F.; Jenner, P.; Marsden, C. D. Ann. Neurol. 1994, 36, 348- 355   DOI   ScienceOn
45 Sagripant, J. L.; Swicord, M. L.; Davis, C. C. Radiat. Res. 1987, 110, 219-231   DOI
46 Imlay, J. A.; Chin, S. M.; Linn, S. Science 1988, 240, 640-642   DOI
47 Brown, C. E. J. Theorl. Biol. 1981, 88, 245-256   DOI
48 Ilic, T.; Jovanovic, M.; Jovicic, A.; Tomovic, M. Vojnosanit. Pregl. 1998, 55, 463-468
49 Jakes, R.; Spillantini, M. G.; Goedert, M. FEBS Lett. 1994, 345, 27-32   DOI   ScienceOn