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http://dx.doi.org/10.5012/bkcs.2005.26.3.413

Fluorescence Quenching of Green Fluorescent Protein during Denaturation by Guanidine  

Jung, Ki-Chul (Department of Chemistry, Chungnam National University)
Park, Jae-Bok (Department of Chemistry, Chungnam National University)
Maeng, Pil-Jae (Department of Microbiology, Chungnam National University)
Kim, Hack-Jin (Department of Chemistry, Chungnam National University)
Publication Information
Bulletin of the Korean Chemical Society / v.26, no.3, 2005 , pp. 413-417 More about this Journal
Abstract
Fluorescence of green fluorescent protein mutant, 2-5 GFP is observed during denaturation by guanidine. The fluorescence intensity decreases exponentially but the fluorescence lifetime does not change during denaturation. The fluorescence lifetime of the denatured protein is shorter than that of native form. As the protein structure is modified by guanidine, solvent water molecules penetrate into the protein barrel and protonate the chromophore to quench fluorescence. Most fluorescence quenchers do not affect the fluorescence of native form but accelerate the fluorescence intensity decay during denaturation. Based on the observations, a simple model is suggested for the structural change of the protein molecule during denaturation.
Keywords
GFP; Fluorescence; Denaturation; Quencher;
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1 Kummer, A. G.; Kompa, C.; Niwa, H.; Hirano, T.; Kojima, S.; Michel-Beryerle, M. E. J. Phys. Chem. B 2002, 106, 7554   DOI   ScienceOn
2 Yazal, J. E.; Prendergast, F. G.; Shaw, D. E.; Pang, Y.-P. J. Am. Chem. Soc. 2000, 122, 11411   DOI   ScienceOn
3 Fukuda, H.; Arai, M.; Kuwajima, K. Biochemistry 2000, 39, 12025   DOI   ScienceOn
4 Tsien, R. Annu. Rev. Biochem. 1998, 67, 509   DOI   ScienceOn
5 Zimmer, M. Chem. Rev. 2002, 102, 759   DOI   ScienceOn
6 Chalfie, M.; Tu, Y.; Euskirchen, G.; Ward, W. W.; Prasher, D. C. Science 1994, 263, 802   DOI
7 Yang, F.; Moss, L.; Phillilps, G. Nature Biotechnol. 1996, 14, 1246   DOI   ScienceOn
8 Ormo, M.; Cubitt, A. B.; Kallio, K.; Gross, L. A.; Tsien, R. Y.; Remington, S. J. Science 1996, 273, 1392   DOI   ScienceOn
9 Heim, R.; Prasher, D. C.; Tsien, R. Y. Proc. Natl. Acad. Sci. USA 1994, 91, 12501   ScienceOn
10 Aune, K. C.; Tanford, C. Biochemistry 1969, 8, 4579   DOI   ScienceOn
11 Mazon, H.; Marcillat, O.; Forest, E.; Smith, D. L.; Vial, C. Biochemistry 2004, 43, 5045   DOI   ScienceOn
12 Siemering, K. R.; Golbik, R.; Sever, R.; Haseloff, J. Curr. Biol. 1996, 6, 1653   DOI   ScienceOn
13 Heim, R.; Cubitt, A. B.; Tsien, R. Y. Nature 1995, 373, 663
14 Swaminathan, R.; Hoang, C. P.; Verkman, A. S. Biophysical J. 1997, 72, 1900   DOI   ScienceOn
15 Prasher, D. C.; Eckenrode, V. K.; Ward, W. W.; Prendergast, F. G.; Cormier, M. J. Gene 1992, 111, 229   DOI   ScienceOn
16 Chattoraj, M.; King, B. A.; Bublitz, G. U.; Boxer, S. G. Proc. Natl. Acad. Sci. USA 1996, 93, 8362   ScienceOn
17 Ward, W. W.; Bokman, S. H. Biochemistry 1982, 21, 4535   DOI   ScienceOn
18 Cubitt, A. B.; Wollenweber, L. A.; Heim, R. Methods Cell Biol. 1999, 6, 19
19 Beringhelli, T.; Eberini, I.; Galliano, M.; Pedoto, A.; Perduca, M.; Sportiello, A.; Fontana, E.; Monaco, H. L.; Gianazza, E. Biochemistry 2002, 41, 15415   DOI   ScienceOn
20 Lee, M.; Park, S. K.; Chung, C.; Kim, H. Bull. Korean Chem. Soc. 2004, 25, 1031   DOI   ScienceOn
21 Lackowicz, J. R. Principles of Fluorescence Spectroscopy, 2nd Ed.: Kluwer: New York, 1999
22 Palm, G. J.; Zdanov, A.; Gaitanaris, G. A.; Stauber, R.; Pavlakis, G. N.; Wlodawer, A. Nature Struc. Biol. 1997, 4, 361   DOI   ScienceOn
23 Ehrhardt, D. Curr. Opin. Plant Biol. 2003, 6, 622   DOI   ScienceOn
24 Ward, W. W.; Prentice, H. J.; Roth, A. F.; Cody, C. W.; Reeves, S. C. Photochem. Photobiol. 1982, 35, 803   DOI
25 Cubitt, A. B.; Heim, R.; Adams, S. R.; Boyd, A. E.; Gross, L. A.; Tsien, R. Y. Trends Biochem. Soc. 1995, 20, 448   DOI   ScienceOn
26 van Roessel, P.; Brand, A. H. Nature Cell Biology 2002, 4, E15   DOI   ScienceOn