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http://dx.doi.org/10.5012/bkcs.2005.26.1.151

Conformational Dynamics of Heme Pocket in Myoglobin and Hemoglobin  

Kim, Seong-Heun (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University)
Heo, Jeong-Hee (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University)
Lim, Man-Ho (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University)
Publication Information
Bulletin of the Korean Chemical Society / v.26, no.1, 2005 , pp. 151-156 More about this Journal
Abstract
The conformational dynamics of heme pocket, a small vacant site near the binding site of heme proteins -myoglobin (Mb) and hemoglobin (Hb), was investigated after photolysis of carbon monoxide from MbCO and HbCO in D$_2$O solution at 283 K by probing time-resolved vibrational spectra of photolyzed CO. Two absorption bands, arising from CO in the heme pocket, evolve nonexponentially in time. The band at higher energy side blue shifts and broadens with time and the one at lower energy side narrows significantly with a negligible shift. These spectral evolutions are induced by protein conformational changes following photolysis that modify structure and electric field of heme pocket, and ligand dynamics in it. The conformational changes affecting the spectrum of photolyzed CO in heme pocket likely modulates ligand-binding activity.
Keywords
Conformational dynamics; Myoglobin; Hemoglobin; Time-resolved IR spectroscopy; Heme pocket;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 12  (Related Records In Web of Science)
Times Cited By SCOPUS : 14
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1 Springer, B. A.; Sligar, S. G.; Olson, J. S.; Phillips, G. N., Jr. Chem. Rev. 1994, 94, 699   DOI   ScienceOn
2 Stryer, L. Biochemistry; W. H. Freeman and Co.: San Francisco, 1988; Vol. 3rd
3 Swiss-Prot Protein Database; National Center of Biotechnology Information: Bethesda, 1994
4 Lim, M.; Jackson, T. A.; Anfinrud, P. A. J. Chem. Phys. 1995, 102, 4355   DOI   ScienceOn
5 Lim, M.; Jackson, T. A.; Anfinrud, P. A. Science 1995, 269, 962   DOI   ScienceOn
6 Lim, M.; Jackson, T. A.; Anfinrud, P. A. Nature Struct. Biol. 1997, 4, 209   DOI   ScienceOn
7 Phillips, S. E. V. J. Mol. Biol. 1980, 142, 531   DOI
8 Shaanan, B. J. Mol. Biol. 1983, 171, 31   DOI
9 Agmon, N.; Hopfield, J. J. J. Chem. Phys. 1983, 79, 2042   DOI
10 Kuczera, K.; Lambry, J. C.; Martin, J. L.; Karplus, M. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5805   ScienceOn
11 Lim, M.; Jackson, T. A.; Anfinrud, P. A. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5801   ScienceOn
12 Hagen, S. J.; Hofrichter, J.; Eaton, W. A. J. Phys. Chem. 1996, 100, 12008   DOI   ScienceOn
13 Deak, J.; Chiu, H.; Lewis, C. M.; Miller, R. J. D. J. Phys. Chem. B 1998, 102, 6621   DOI   ScienceOn
14 Findsen, E. W.; Scott, T. W.; Chance, M. R.; Friedman, J. M.; Ondrias, M. R. J. Am. Chem. Soc. 1985, 107, 3355   DOI
15 Causgrove, T. P.; Dyer, R. B. Biochemistry 1993, 32, 11985   DOI   ScienceOn
16 Causgrove, T. P.; Dyer, R. B. J. Phys. Chem. 1996, 100, 3273   DOI   ScienceOn
17 Kim, S.; Jin, G.; Lim, M. Bull. Korean Chem. Soc. 2003, 24, 1470   DOI   ScienceOn
18 Brunori, M.; Vallone, B.; Cutruzzola, F.; Travaglini-Allocatelli, C.; Berendzen, J.; Chu, K.; Sweet, R. M.; Schlichting, I. Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 2058   ScienceOn
19 Schott, F.; Lim, M.; Jackson, T. A.; Smirnov, A.; Soman, J.; Olson, J. S.; George, N.; Phillips, J.; Wulff, M.; Anfinrud, P. A. Science 2003, 300, 1944   DOI   ScienceOn
20 Chu, K.; Vojtchovsky, J.; McMahon, B. H.; Sweet, R. M.; Berendzen, J.; Schlichting, I. Nature 2000, 403, 921   DOI   ScienceOn
21 Lim, M.; Jackson, T. A.; Anfinrud, P. A. J. Am. Chem. Soc. 2004, 126, 7946   DOI   ScienceOn
22 Anfinrud, P. A.; Lim, M.; Jackson, T. A. Proc. SPIE-Int. Soc. Opt. Eng. (Longer Wavelength Lasers and Applications) 1994, 2138, 107
23 Kim, S.; Jin, G.; Lim, M. J. Phys. Chem. B 2005, ASAP
24 Hamm, P.; Lim, L.; Hochstrasser, R. M. J. Phys. Chem. B 1998, 102, 6123   DOI   ScienceOn
25 Alben, J. O.; Beece, D.; Bowne, S. F.; Doster, W.; Eisenstein, L.; Frauenfelder, H.; Good, D.; McDonald, J. D.; Marden, M. C.; Mo, P. P.; Reinisch, L.; Reynolds, A. H.; Shyamsunder, E.; Yue, K. T. Proc. Natl. Acad. Sci. U.S.A. 1982, 79, 3744   ScienceOn
26 Anfinrud, P. A.; Han, C.; Hochstrasser, R. M. Proc. Natl. Acad. Sci. U.S.A. 1989, 86, 8387   ScienceOn
27 Petrich, J. W.; Poyart, C.; Martin, J. L. Biochemistry 1988, 27, 4049   DOI   ScienceOn
28 Henry, E. R.; Sommer, J. H.; Hofrichter, J.; Eaton, W. A. J. Mol. Biol. 1983, 166, 443   DOI
29 Lian, T.; Locke, B.; Kholodenko, Y.; Hochstrasser, R. M. J. Phys. Chem. 1994, 98, 11648   DOI   ScienceOn
30 Venyaminov, S. Y.; Prendergast, F. G. Anal. Biochem. 1997, 248, 234   DOI   ScienceOn
31 Li, P.; Champion, P. M. Biophys. J. 1994, 66, 430   DOI   ScienceOn
32 Lim, M.; Jackson, T. A.; Anfinrud, P. A. J. Phys. Chem. 1996, 100, 12043   DOI   ScienceOn
33 Lingle, R., Jr.; Xu, X.; Zhu, H.; Yu, S. C.; Hopkins, J. B. J. Phys. Chem. 1991, 95, 9320   DOI
34 Lingle, R., Jr.; Xu, X.; Zhu, H.; Yu, S. C.; Hopkins, J. B.; Straub, K. D. J. Am. Chem. Soc. 1991, 113, 3992   DOI
35 Brooks, C. L., III; Karplus, M.; Pettitt, B. M. Proteins: a Theoretical Perspective of Dynamics, Structure and Thermodynamics; John Wiley & Sons: New York, 1988
36 Jackson, T. A.; Lim, M.; Anfinrud, P. A. Chem. Phys. 1994, 180, 131   DOI   ScienceOn
37 Tian, W. D.; Sage, J. T.; Srajer, V.; Champion, P. M. Phys. Rev. Lett. 1992, 68, 408   DOI   ScienceOn
38 McMahon, M. T.; deDios, A. C.; Godbout, N.; Salzmann, R.; Laws, D. D.; Le, H.; Havlin, R. H.; Oldfield, E. J. Am. Chem. Soc. 1998, 120, 4784   DOI   ScienceOn
39 Rudolph, S. A.; Boyle, S. O.; Dresden, C. F.; Gill, S. J. Biochemistry 1972, 11, 1098   DOI   ScienceOn
40 Srajer, V.; Champion, P. M. Biochemistry 1991, 30, 7390   DOI   ScienceOn
41 Genberg, L.; Richard, L.; McLendon, G.; Miller, R. J. D. Science 1991, 251, 1051   DOI
42 Steinbach, P. J.; Ansari, A.; Berendzen, J.; Braunstein, D.; Chu, K.; Cowen, B. R.; Ehrenstein, D.; Frauenfelder, H.; Johnson, J. B. et al. Biochemistry 1991, 30, 3988   DOI   ScienceOn
43 Petrich, J. W.; Lambry, J. C.; Kuczera, K.; Karplus, M.; Poyart, C.; Martin, J. L. Biochemistry 1991, 30, 3975   DOI   ScienceOn
44 Perutz, M. F.; Mathews, F. S. J. Mol. Biol. 1966, 21, 199   DOI
45 Luisi, B.; Liddington, B.; Fermi, G.; Shibayama, N. J. Mol. Biol. 1990, 214, 7   DOI
46 Lim, M.; Wolford, M. F.; Hamm, P.; Hochstrasser, R. M. Chem. Phys. Lett. 1998, 290, 355   DOI   ScienceOn
47 Murray, L. P.; Hofrichter, J.; Henry, E. R.; Eaton, W. A. Biophy. Chem. 1988, 29, 63   DOI   ScienceOn
48 Hamm, P.; Kaindl, R. A.; Stenger, J. Opt. Lett. 2000, 25, 1798   DOI   ScienceOn
49 Lim, M. Bull. Korean Chem. Soc. 2002, 23, 865   DOI   ScienceOn
50 Ansari, A.; Jones, C. M.; Henry, E. R.; Hofrichter, J.; Eaton, W. A. Biochemistry 1994, 33, 5128   DOI   ScienceOn
51 Meuwly, M.; Becker, O. M.; Stote, R.; Karplus, M. Biophys. Chem. 2002, 98, 183   DOI   ScienceOn
52 Takano, T. J. Mol. Biol. 1977, 110, 569   DOI