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http://dx.doi.org/10.5012/bkcs.2002.23.7.985

Purification and Characterization of a Laccase from Cerrena unicolor and Its Reactivity in Lignin Degradation  

Kim, You-Sung
Cho, Nam-Seok
Eom, Tae-Jin
Shin, Woon-Sup
Publication Information
Bulletin of the Korean Chemical Society / v.23, no.7, 2002 , pp. 985-989 More about this Journal
Abstract
For efficient biopulping process, very active and stable lignase is essential. Laccase is one of the best enzyme in terms of environmentally benign processes, since the enzyme uses oxygen as an oxidant to degrade lignin and produces no hamful prod ucts. We could purify a laccase homogeneously from Cerrena unicolor in a very active state. It shows characteristic absorption feature with blue band at λmax = 604 ㎚. Molecular weight of the enzyme is 57,608 which could be accurately determined by MALDI/TOF MS. The enzyme has 2.8 copper ions per enzyme implying apoenzymes might exist together. The enzyme is active in lignin degradation and the activity increases 4 times in the presence of ABTS as a mediator.
Keywords
Laccase; Cerrena unicolor; Purification; Lignin; Degradation.;
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[ Addleman, K.;Dumonceaux, T.;Paice, M. G.;Bourbonnais, R.;Archibald, F. S. ] / Appl. Environ. Microbiol.
2 Pammer, J. M.; Harvey, P. J.; Shoemaker, H. E.; Schmidt, H. W.H.; Leisola, M. S. A. FEBS Lett. 1987, 220, 149.   DOI   ScienceOn
3 Xu, F.; Shin, W.; Brown, S. H.; Wahleithner, J.; Sundaram, U. M.;Solomon, E. I. Biochim. Biophys. Acta 1996, 1292, 303.   DOI   ScienceOn
4 Bekker, E. G.; Petrova, S. D.; Ermolova, D. V.; Elisashvili, V. I.;Sillnitsyn, A. P. Biokhimiya 1990, 55, 2019.
5 Smook, G. A. Handbook for Paper and Pulp Technologists;TAPPI: Atlanta, 1989.
6 Jung, H.; Xu, F.; Li, K. Enzyme Microb. Technol. 2002, 30, 161.   DOI   ScienceOn
7 Leontievsky, A.; Myasoedova, N.; Polzdnyakova, N.; Golovleva, L. FEBS Lett. 1997, 413, 446.   DOI   ScienceOn
8 Hyung, K. H.; Jun, K. Y.; Hong, H.- G.; Kim, H. S.; Shin, W. Bull. Korean Chem. Soc. 1997, 18, 564.
9 Bourbonnais, R.; Paice, M. G.; Reid, I. D.; Lanthier, P.; Yaguchi, M. Appl. Environ. Microbiol. 1995, 61, 1876.
10 Bajpai, P.; Bajpai, P. K.; Kondo, R. Biotechnology for EnvironmentalProtection in the Pulp and Paper Industry; SpringerVerlag: Berlin, 1999; p 49.
11 Christov, L. P.; Prior, B. A. Enzymes for Pulp and Paper Processing; Jeffries, T. W., Viikari, L., Eds.; American Chemical Society; Washington, 1996; p 208.
12 Subramaniyan, S.; Prema, P. FEMS Microbiol. Lett. 2000, 183, 1.   DOI   ScienceOn
13 Kuwahara, M.; Glenn, J. K.; Morgan, M. A.; Gold, M. H. FEBS Lett. 1984, 169, 247.   DOI   ScienceOn
14 Call, H. P.; Mucke, I. J. Biotechnol. 1997, 53, 163.   DOI   ScienceOn
15 Yaropolov, A. I.; Skorobogatko, O. V.; Vartanov, S. S.; Varfolomeyev, S. D. Appl. Biochem. Biotechnol. 1994, 49, 257.   DOI   ScienceOn
16 Palmore, G. T. R.; Kim, H.-H. J. Electroanal. Chem. 1999, 464, 110.   DOI   ScienceOn
17 Ramasamy, K.; Kelley, R. L.; Reddy, C. A. Biochem. Biophys. Res. Commun. 1985, 131, 436.   DOI   ScienceOn
18 Sealey, J. E.; Runge, T. M.; Ragauskas, A. J. Proceedings of Biological Sciences Symposium; TAPPI Proceedings: 1997; p 339.
19 Bajpai, P.; Bajpai, P. K.; Kondo, R. Biotechnology for EnvironmentalProtection in the Pulp and Paper Industry; SpringerVerlag: Berlin, 1999; p 29.
20 Kuhad, R. C.; Singh, A.; Eriksson, K.-E. L. Biotechnology in the Pulp and Paper Industry; Springer Verlag: Berlin, 1997; p 45.
21 Trojanowski, J.; Leonowicz, A.; Hampel, B. Acta Microbiol.Polon. 1966, 15, 17.
22 Bourbonnais, R.; Paice, M. G. Tappi J. 1996, 79, 199.
23 Solomon, E. I.; Sundaram, U. M.; Machonkin, T. E. Chem. Rev. 1996, 96, 2563.   DOI   ScienceOn
24 Li, K.; Xu, F.; Eriksson, K.-E. L. Appl. Environ. Microbiol. 1999,65, 2654.
25 Kirk, T. K.; Jeffries, T. W. Enzymes for Pulp and PaperProcessing; Jeffries, T. W., Viikari, L., Eds.; American ChemicalSociety; Washington, 1996; p 2.
26 Skoog, D. A.; Holler, F. J.; Nieman, T. A. Principles of InstrumentalAnalysis; Saunders College Publishing: Philadelphia, 1998;p 498.
27 Paszczynski, A.; Huynh, V.-B.; Crawford, R. FEMS Microbiol. Lett. 1985, 29, 37.   DOI
28 Bourbonnais, R.; Paice, M. G.; Freiermuth, B.; Bodie, E.;Borneman, S. Appl. Environ. Microbiol. 1997, 63, 4627.
29 Cho, N.-S.; Ohga, S.; Pashenova, N.; Leonowicz, A. Proc. 2ndInternatl Sym. For. Sci.; Chungbuk Nat. Univ.: Cheongju, Korea,2000; pp 132.
30 Yaver, D. S.; Overjero, M. D. C.; Xu, F.; Nelson, B. A.; Brown, K. M.; Halkier, T.; Bernauer, S. Appl. Environ. Microbiol. 1999, 65, 4943.
31 Ghindilis, A. L.; Gavrilova, V. P.; Yaropolov, A. I. Biosens. Bioelectron. 1992, 7, 127.   DOI   ScienceOn
32 Bollag, J.-M.; Leonowicz, A. Appl. Environ. Microbiol. 1984, 48,849.
33 Gianfreda, L.; Sannino, F.; Filazzola, M. T.; Leonowicz, A. J. Mol. Cat. B 1998, 4, 13.   DOI   ScienceOn
34 Barton, S. C.; Kim, H.-H.; Binyamin, G.; Zhang, Y.; Heller, A. J. Phys. Chem. B 2001, 105, 11917.   DOI   ScienceOn
35 Heinzkill, M.; Bech, L.; Halkier, T.; Schneider, P.; Anke, T. Appl. Environ. Microbiol. 1998, 64, 1601.
36 Rogalski, J.; Dawidowicz, A.; Jozwik, E.; Leonowicz, A. J. Mol. Cat. B 1999, 6, 29.   DOI   ScienceOn
37 Dean, J. F. D.; Eriksson, K.-E. L. Holzforschung 1994, 48, 21.   DOI   ScienceOn
38 Srinivasan, C.; Dsuoza, T. M.; Boominathan, K.; Reddy, C. A. Appl. Environ. Microbiol. 1995, 61, 4174.
39 Solomon, E. I.; Baldwin, M. J.; Lowery, M. D. Chem. Rev. 1992,92, 521.   DOI
40 Hanna, P. M.; Tamilarasan, R.; McMillin, D. R. Biochem. J. 1988, 256, 1001.
41 Felsenfeld, G. Arch. Biochem. Biophys. 1960, 87, 247.   DOI   ScienceOn
42 Bourbonnais, R.; Paice, M. G. Appl. Microbiol. Biotechnol. 1992, 36, 823.
43 Wojtas-Wasilewska, M.; Luterpek, J.; Rogalski, J. Phytochem.1988, 27, 2731.   DOI   ScienceOn
44 Pelly, J. W.; Garner, C. W.; Little, G. H. Anal. Biochem. 1978, 86,341.   DOI   ScienceOn
45 Ni, Y.; Kubes, G. J.; Van Heinigen, A. R. P. J. Pulp Pap. Sci. 1990,16, J83.
46 Hyung, K. H.; Shin, W. J. Korean Electrochem. Soc. 1999, 2, 31.
47 Addleman, K.; Dumonceaux, T.; Paice, M. G.; Bourbonnais, R.; Archibald, F. S. Appl. Environ. Microbiol. 1995, 61, 3687.
48 Barton, S. C.; Kim, H.-H.; Binyamin, G.; Zhang, Y.; Heller, A. J. Am. Chem. Soc. 2001, 123, 5802.   DOI   ScienceOn
49 Temp, U.; Eggert, C. Appl. Environ. Microbiol. 1999, 65, 389.
50 Quan, D.; Kim, Y.; Yoon, K. B.; Shin, W. Bull. Korean Chem. Soc. 2002, 23, 385.   DOI   ScienceOn
51 Wahleithner, J. A.; Xu, F.; Brown, K. M.; Brown, S. H.; Gotightly,E. J.; Halkier, T.; Kauppimen, S.; Pederson, A.; Schneider, P. Curr.Genet. 1996, 29, 395.   DOI   ScienceOn
52 Breen, A.; Singleton, F. L. Curr. Opin. Biotechnol. 1999, 10, 252.   DOI   ScienceOn