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http://dx.doi.org/10.5012/bkcs.2002.23.10.1399

Development of an Enzyme-Linked Immunosorbent Assay for the Organophorus Insecticide Bromophos  

Park, Won-Cheol
Cho, Young-Ae
Kim, Yoo-Jung
Hammock, Bruce D.
Lee, Yong-Tae
Lee, Hye-Sung
Publication Information
Bulletin of the Korean Chemical Society / v.23, no.10, 2002 , pp. 1399-1426 More about this Journal
Abstract
A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative detection of the organophosphorus insecticide bromophos. Three bromophos analogues (haptens) were synthesized and were coupled to carrier proteins to use as immunogens or coating antigens. Rabbits were immunized with either one of two haptens coupled to bovine serum albumin (BSA) for production of polyclonal antibodies, and the sera were screened against one of the haptens coupled to ovalbumin (OVA). Using the serum with highest specificity and an enzyme tracer, an antibody-coated ELISA was developed, which showed an $IC_{50}$ of 40 ng/mL with a detection limit of 7 ng/mL. The antibodies in this assay showed negligible cross-reactivity with other organophosphorus pesticides except with the insecticides chlorpyrifos and fenitrothion.
Keywords
Bromophos; Insecticide; Immunoassay; ELISA;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
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1 Lee, J. G.; Ahn, K. C.; Park, O. S.; Kang, S. Y.; Hammock, B. D. J. Agric. Food Chem. 2001, 49, 2159-2167.   DOI   ScienceOn
2 Skerritt, J. H.; Lee, N. J. In Residue Analysis in Food Safety: Applications of Immunoassay Methods; Beier R. C., Stanker, L. H., Eds.; ACS Symposium Series 621; American Chemical Society: Washington DC, U. S. A., 1996; pp 124-149.
3 Park, H. J.; Park, W. C.; Jung, T.; Rha, C. S.; Lee, Y. T. Bull. Korean Chem. Soc. 2002, 23, 599-604.   DOI   ScienceOn
4 Tabor, E. J. US Patent 1961, 3,005,005.
5 Manclús, J. J.; Primo, J.; Montoya, A. J. Agric. Food Chem. 1996, 44, 4052-4062.   DOI   ScienceOn
6 Raab, G. M. Clin. Chem. 1983, 29, 1757-1761.
7 Hill, A. S.; McAdam, D. P.; Edward, S. L.; Skerritt, J. H. J. Agric. Food Chem. 1993, 41, 2011-2018.   DOI   ScienceOn
8 Jung, F.; Gee, S. J.; Harrison, R. O.; Goodrow, M. H.; Karu, A. E.; Braun, A. L.; Li, Q. X.; Hammock, B. D. Pestic. Sci. 1989, 26, 303-317.
9 AOAC International. Official Methods of Analysis, 18th ed.; AOAC International: Arlington, VA, 1995; Section 970.52
10 Hassner, A.; Alexanian, V. Tetrahedron Lett. 1978, 46, 4475-4478.
11 McAdam, D. P.; Skerritt, J. H. Aust. J. Chem. 1993, 46, 959-967.   DOI   ScienceOn
12 McAdam, D. P.; Hill, A. S.; Beasley, H. L.; Skerritt, J. H. J. Agric. Food Chem. 1992, 40, 1466-1470.   DOI
13 Hennion, M. C.; Barcelo, D. Anal. Chim. Acta 1998, 362, 3-34.   DOI   ScienceOn
14 Edward, S. L.; Skerritt, J. H.; Hill, A. S.; McAdam, D. P. Food Agric. Immunol. 1993, 5, 129-144.   DOI   ScienceOn
15 Park, J. H.; Kim, Y. J.; Lee, Y. T. Bull. Korean Chem. Soc. 2002, 23, 605-609.   DOI   ScienceOn
16 Cho, Y. A.; Lee, H.-S.; Park, E. Y.; Lee, Y. T.; Hammock, B. D.; Ahn, K. C.; Lee, J. K. Bull. Korean Chem. Soc. 2002, 23, 481-487.   DOI   ScienceOn
17 Hassall, K. A. The Biochemistry and Uses of Pesticides, VCH: New York, 1990; p 122.
18 Heldman, E.; Balan, A.; Horowitz, O.; Ben-Zion, S.; Torten, M. FEBS Lett. 1985, 180, 243-248.   DOI   ScienceOn
19 Ross, F.; Park, V. III. US patent 1973, 3,728,403.
20 Eichler, D. Residue Rev. 1972, 41, 65-112.
21 Hill, A. S.; Skerritt, J. H.; Bushway, R. J.; Perkins, L. B.; Larkin, K.; Thomas, M.; Korth, W.; Bowmer, K. H. J. Agric. Food Chem. 1994, 42, 2051-2058.   DOI   ScienceOn
22 Skerritt, J. H.; Hill, A. S.; Thwaites, H. L.; Edward, S. L.; McAdam, D. P. J. AOAC Int. 1992, 73, 519-528.
23 Marco, M. P.; Gee, S. J.; Hammock, B. D. Trends Anal. Chem. 1995, 14, 415-425.