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http://dx.doi.org/10.7730/JSCM.2012.24.3.80

Effects of Korean Herbal Bathing Extracts Composition on Th2 Cytokine Production in MC/9 Mast Cells  

Jang, Moon-Hee (Dept. of Sasang Constitutional Medicine, College Oriental Medicine, Daejeon Univ.)
Choi, Jae-Song (Dept. of Sasang Constitutional Medicine, College Oriental Medicine, Daejeon Univ.)
Choi, Ae-Ryun (Dept. of Sasang Constitutional Medicine, College Oriental Medicine, Daegu Haany Univ.)
Ahn, Taek-Won (Dept. of Sasang Constitutional Medicine, College Oriental Medicine, Daejeon Univ.)
Publication Information
Journal of Sasang Constitutional Medicine / v.24, no.3, 2012 , pp. 80-92 More about this Journal
Abstract
Objectives The purpose of this study is to investigate the effects of korean herbal bathing extracts composition 1 and composition 2 on Th2 cytokine production in MC/9 mast cells. Methods The effects of composition 1, 2 was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. Results Composition 1, 2 inhibited the IL-5, IL-13 production significantly(p<.001) in comparison to PI-control group at concentration of 100 ${\mu}g/mL$, 200 ${\mu}g/mL$. Composition 1, 2 inhibited the IL-4, IL-5, IL-13 mRNA expression significantly in comparison to PI-control group at concentration of 100 ${\mu}g/mL$, 200 ${\mu}g/mL$. Composition 1 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 200 ${\mu}g/ml$. Composition 2 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 100 ${\mu}g/mL$, 200 ${\mu}g/mL$. Conclusions These results indicate that composition 1, 2 has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.
Keywords
Korean Herbal Bathing Extracts Composition; Cytokine; MC/9 Mast Cell; Atopic Dermatitis;
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Times Cited By KSCI : 5  (Citation Analysis)
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