Browse > Article

Stimulation of the Immune Response by Herbal Formulas for Taeeumin  

Jung, Da-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Ha, Hye-Kyung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Lee, Ho-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Lee, Jin-Ah (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Lee, Nam-Hun (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Lee, Jun-Kyoung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Huang, Dae-Sun (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Publication Information
Journal of Sasang Constitutional Medicine / v.22, no.3, 2010 , pp. 141-151 More about this Journal
Abstract
1. Objectives: Three herbal formulas (Yuldahanso-tang, Chungsimyonja-tang, and Taeeumjowi-tang) for Taeeumin were applied to investigate the immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses in murine macrophage cell line (RAW 264.7) and ovalbumin (OVA)-immunized mice. 2. Methods: This study was carried out in nitric oxide (NO) synthesis in RAW 264.7 cells and cellular proliferation in mouse splenocytes according to three herbal formulas. C57BL/6 mice were immunized intraperitonially with OVA/aluminium ($100\;{\mu}g/200\;{\mu}g$/mouse) on day 1, 8, and 15. Three herbal formulas were administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, IgG1, and total IgM classes) in plasma were measured. 3. Results: Yuldahanso-tang and Chungsimyonja-tang increased NO synthesis in RAW 264.7 cells. Three herbal formulas significantly enhanced cellular proliferation by LPS and Con A in splenocytes from OVA-immunized mice (p<.001). Three herbal formulas for Taeeumin also significantly enhanced plasma OVA-specific IgG, IgG1, and total IgM levels compared with the OVA/Alum group. 4. Conclusion: These results suggested that three herbal formulas for Taeeumin could be used as stimulator of immune response.
Keywords
Yuldahanso-tang (Reduohanshao-tang); Chungsimyonja-tang (Qingxinlianzi-tang); Taeeumjowi-tang (Taiyintiaoweitang); Immune response;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Cyster JG. Chemokines and cell migration in secondary lymphoid organs. Science. 1999;286(5447):2098-2102.   DOI   ScienceOn
2 Dutton RW, Bradley LM, Swain SL. T cell memory. Annu Rev Immnol. 1998;16:201-223.   DOI   ScienceOn
3 Kupper TS. T cells, immnonsurveillance, and cutaneous immunity. J Dermatol Sci. 2000;24:S41-S45.   DOI   ScienceOn
4 Kim KS, Lee DU, Kim YL, Hwang MJ, Kim GW, Koo BS. Anti-obesity and anti-hyperlipidemic effects of Taeyeumjowee-tang and its modified prescription. J Oriental Neuropsyshiatry. 2007;18(2):57-74. (Korean)   과학기술학회마을
5 Lee JH, Park HS, Kim KY. Effects of Yuldahansotang water ectract on cultured primary hippocampal cell culture damaged by hydrogen peroxide. J Sasang Constitut Med. 2002;14(1):79-89. (Korean)   과학기술학회마을
6 Hong JH, Kim KW, Yu DG, Park HS, Kim HS. Effects of Yuldahansotang water extract on cultured spinal sensory neurons damaged by xanthine oxidase/ hypoxanthine. J Sasang Constitut Med. 2001;13(1):88-96. (Korean)
7 Chou LS, Ahn TW, Lee H. Studies of Chengsimyeonja -tang and Taeeumjowi-tang that get change by Wistar rat's aging. J Sasang Constitut Med. 2005;17(2): 74-84. (Korean)   과학기술학회마을
8 Kim MW, Park SS. Effects of Taeeumjowetang on lipid peroxidation by free radicals and oxidative damage of hepatocytes by tert-butyl hybroperoxide. J Sasang Constitut Med. 2001;13(1):51-60. (Korean)
9 Lim JP, Ahn TW. The anti-oxiative and immune-regluatory effect of Chungsimyeonja-tang in aged rat. J Sasang Constitut Med. 2007;19(3):227-241. (Korean)   과학기술학회마을
10 Park SC. Effects CheongSimYeonJaTang(CSYJT) on control of immune-function in highly purified mouse B cells and Mast cell. J Sasang Constitut Med. 2003; 15(2):166-179. (Korean)   과학기술학회마을
11 Hong SC, Ko BH, Song IB. An experimental study on the anti-stress effects by taeeumin Chongsimyonjat ang. J Sasang Constitut Med. 1995;7(2):227-240. (Korean)
12 Liew FY, Lia Y, Millot S. Tumor necrosis factor synergizes with IFN-γ in mediating killing of Leishmania major through the induction of nitric oxide. J Immunol. 1990:145:4306-4310.
13 Higuchi M, Higashi N, Taki H, Osawa T. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanism of activated macrophages. J Immunol. 1990;144(4):1425-1431.
14 Hur SM, Kwon CH, Joh JW, Kim SJ. Alteration of molecules and cytokines related to the activation of T lymphocyte in immune tolerance induced mice model. J Korean Surg Soc. 2005;19(2):119-123. (Korean)
15 Kawamata H, Ochiai H, Mantani N, Terasawa. Enhanced expreesion of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW 264.7 cells, a murine macrophage cell line. Am J Chin Med, 2000;28:217-226.   DOI   ScienceOn
16 Kim KY. Consideration about medicine historical view of Je-Ma Lee. J Sasang Constitut Med. 1994;6(1): 11-19. (Korean)
17 Cho DW, Cho HS, Cho CS. Genetic analysis study of sasang constitution classification by DNA-fingerprinting methods. J Sasang Constitut Med. 1996;8(2): 151-163. (Korean)
18 Seo YB, Kil GJ, Lee YC, Kim DH. Experimental studies on thymus cells differentiation by the extracts of Stigma Croci. Kor J Herbology. 2003;18(3):97-111. (Korean)   과학기술학회마을
19 Jang SJ, Chun HN, Yun SS, Lee IS, Lee YS. Effects of deer antler extract on serum IGF-I, bone growth and splenocyte proliferation in growing rats. Korean J Nutr. 2006;39(3):225-235. (Korean)   과학기술학회마을
20 Friedl R, Moeslinger T, Kopp B, and Spiecke- rmann PG. Stimulation of nitric oxide synthesis by the aqueous extract of Panax ginsena root in Raw 264.7 cells. Br J pharmacol. 2001;134:1663-1670.   DOI   ScienceOn
21 Farrell AJ, Blake DR. Nitric oxide. Annals of the Rheumatic Dieases. 1996;55:7-20.   DOI   ScienceOn
22 Ryu HS, Kim J, Park SC, Kim HS. Enhancing effect of Zingiber Officinale Roscoe extract on mouse spleen and macrophage cells activation. Korean J of Nutrition. 2004;37(9):780-785. (Korean)   과학기술학회마을
23 Sun J, Hu S, Song X. Adjuvant effects protopanaxadiol and protopanaxatriol saponins from ginseng roots on the immune reponses to ovalbumin in mice. Vaccine. 2007;25:1114-1120.   DOI   ScienceOn
24 Choi JS, Kim KY ,Kim HM, Ju JC. Studies on the cytokine production regulation in human astrocytes by Yuldahansotang. J Sasang Constitut Med. 2001; 13(1):61-69. (Korean)
25 Choi YJ, Han JH, Kim KY, Kang SY. Effects of Yuldahansotang water extract on blood pressure, regional cerebral blood flow and pial arterial diamter. J Sasang Constitut Med. 1998;10(1):285-293. (Korean)